Home Destination Vector pEX-N-His-GST
PrecisionShuttle bacterial expression vector with N-terminal His-GST tag, 10 ug
- Upon IPTG induction, ORFs cloned in this vector can be expressed in E.coli (BL21/DE3) as a tagged protein with an N-terminal His-GST tag.
- Detection and purification of the transgene are best done using an anti-His antibody or a glutathione affinity column.
- If the N-terminal tagging interferes with the protein’s function, you can choose the C-terminal His-tagging vector, PS100032
Schematic of the multiple cloning sites: