Drive your transfections full throttle with the
high performance reagent TurboFectin 8.0

TurboFectin 8.0 is a new generation of transfection reagent optimized for nucleic acid delivery into eukaryotic cells. Its proprietary formulation of lipid/histone blend is supplied in 80% alcohol. TurboFectin 8.0 is the recommended transfection reagent for delivery of TrueClone  (for over-expression) and HuSH-29 constructs (shRNA for expression knock-down) in many different cell types. TurboFectin 8.0 has also been optimized and is the preferred reverse transfection reagent in GFC-Transfection Arrays.                                                                                 Application Guide

Hek 293T cells were plated at 10,000 cells per well in a 96-well plate in DMEM with 10% FBS. Cells were transfected with pCMV-luciferase using the optimal concentration of TurboFectin 8.0 or Reagent L Luciferase activity was measured at 24, 48 and 72 hrs post-transfection using BriteLite substrate (Perkin Elmer).

• Maximum protein expression over time!
• Gentle enough for primary cells (click for data).
• Recommended for shRNA-induced silencing (click for data).
• Preferred reagent for reverse transfection assays (click for data).
• Simplified use: Works well in media containing antibiotic and antimycotic agents. Suitable for serum-containing media; no requirement for media changes.
• Wide spectrum: Over 100 cell lines and primary cell types have been successfully transfected with TurboFectin 8.0 (Click for list)
• Affordability: One mL of TurboFectin 8.0 can typically be used for 300-500 transfections of 6-well plate cultures. The cost is less than $0.50 per transfection.