OriGene cDNAs in recent publications

a5ß1-Integrin controls ebolavirus entry by regulating endosomal cathepsins, PNAS, May 2009; 106: 8003 - 8008. [CTSB (CATB)]

BIOPHYSICS AND COMPUTATIONAL BIOLOGY: Crystal structure of human aquaporin 4 at 1.8 Å and its mechanism of conductance, PNAS, May 2009; 106: 7437 - 7442. [AQP4]

Charting the molecular network of the drug target Bcr-Abl, PNAS, May 2009; 106: 7414 - 7419. [SHC1]

Chordin-like 1 and Twisted Gastrulation 1 Regulate BMP Signaling following Kidney Injury, Barry W. Larman, Michele J. Karolak, Derek C. Adams, and Leif Oxburgh, J. Am. Soc. Nephrol., May 2009; 20: 1020 - 1031. [CHRDL1]

Competitive binding of musclin to natriuretic peptide receptor 3 with atrial natriuretic peptide, Shunbun Kita, Hitoshi Nishizawa, Yosuke Okuno, Masaki Tanaka, Atsutaka Yasui, Morihiro Matsuda, Yukio Yamada, and Iichiro Shimomura, J. Endocrinol., May 2009; 201: 287 - 295. [NPR1]

Selective regulation of long-form calcium-permeable AMPA receptors by an atypical TARP Nature Neuroscience 12, 277 - 285 (01 Mar 2009), doi: 10.1038/nn.2266 [CACNG7(TARP7)]

View all citations on TrueClone

37,000 TrueORF cDNA clones:
Express proteins with verifiable tags

Seeing is believing, especially when your goal is to achieve protein over-expression.

Engineered as expression-ready clones with verified ORFs and built-in C-terminal tags, TrueORFs express proteins with tracking tags, allowing easy detection and isolation of expressed proteins. (Learn more..)

ORFWestern

25,000 human and 12,000 mouse clones
5,000 human clones ready for next day delivery
Sequence verified
Transfection-ready
C-terminal Myc-DDK* tag for easy detection
Multiple tagging options through PrecisionShuttle
Easy search with Gene Name, Gene Symbol, Accession Number or Keyword

All TrueORF inserts are housed in a pCMV6-Entry vector (below) :

pcmventryvector

Key elements of the TrueORF Entry Vector Download full sequence

Technical features or TrueORF:

The CMV promoter and a Kozak consensus sequence drive protein expression in mammalian cells.
The C-terminus of the ORF is tagged with both Myc and DDK*
Ten (10) ug of purified plasmid is provided for immediate transfection and expression experiments.
An antibiotic selection cassette (Neo^r/Kan^r) confers resistance to kanamycin in E. coli and neomycin analogs in mammalian cells.
The upstream T7 promoter allows protein expression in cell-free system.
Engineered as the entry vector for the OriGene PrecisionShuttle system.

* Peptide sequence of the DDK-tag (Flag®): N-DYKDDDDK-C Flag® is a registered trademark of Sigma-Aldrich

37,000 TrueORF cDNA clones: Express proteins with verifiable tags

37,000 TrueORF cDNA clones:
Express proteins with verifiable tags