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Over 1000 citations of OriGene cDNA clones
Silencing hsp25/hsp27 Gene Expression Augments Proteasome Activity and Increases CD8+ T-Cell–Mediated Tumor Killing and Memory Responses Cancer Prevention Research, Jan 2012; 5: 122 - 137. [HSPB1 ]
Activation of Androgen Receptor, Lipogenesis, and Oxidative Stress Converged by SREBP-1 Is Responsible for Regulating Growth and Progression of Prostate Cancer Cells Mol. Cancer Res., Jan 2012; 10: 133 - 142. [SREBF1 ]
Androgen Deprivation Causes Epithelial–Mesenchymal Transition in the Prostate: Implications for Androgen-Deprivation Therapy Cancer Res., Jan 2012; 72: 527 - 536. [AR ]
CB2 Cannabinoid Receptors Promote Neural Progenitor Cell Proliferation via mTORC1 Signaling J. Biol. Chem., Jan 2012; 287: 1198 - 1209. [Cnr2 ]
View All Citations >>

 

37,000 TrueORF cDNA clones:
Express proteins with verifiable tags

Seeing is believing, especially when your goal is to achieve protein over-expression.

Engineered as expression-ready clones with verified ORFs and built-in C-terminal tags, TrueORFs express proteins with tracking tags, allowing easy detection and isolation of expressed proteins.  (Learn more..)

ORFWestern

  • 25,000 human and 12,000 mouse clones
  • 5,000 human clones ready for next day delivery
  • Sequence verified
  • Transfection-ready
  • C-terminal Myc-DDK* tag for easy detection
  • Multiple tagging options through PrecisionShuttle
  • Easy search with Gene Name, Gene Symbol, Accession Number or Keyword


All TrueORF inserts are housed in a pCMV6-Entry vector (below) :

 pcmventryvector

Key elements of the TrueORF Entry Vector                                  Download full sequence

Technical features or TrueORF:

  • The CMV promoter and a Kozak consensus sequence drive protein expression in mammalian cells.
  • The C-terminus of the ORF is tagged with both Myc and DDK*
  • Ten (10) ug of purified plasmid is provided for immediate transfection and expression experiments.
  • An antibiotic selection cassette (Neor/Kanr) confers resistance to kanamycin in E. coli and neomycin analogs in mammalian cells.
  • The upstream T7 promoter allows protein expression in cell-free system.
  • Engineered as the entry vector for the OriGene PrecisionShuttle system.

* Peptide sequence of the DDK-tag (Flag®): N-DYKDDDDK-C Flag® is a registered trademark of Sigma-Aldrich
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