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OriGene cDNAs cited in recent published Functional Studies

ERdj4 and ERdj5 Are Required for Endoplasmic Reticulum-associated Protein Degradation of Misfolded Surfactant Protein CMol. Biol. Cell. June 2008; 19(6): p. 2620-2630 [DNAJB11]


Protein Kinase C{alpha}-Induced Derepression of the Human Luteinizing Hormone Receptor Gene Transcription through ERK-Mediated Release of HDAC1/Sin3A Repressor Complex from Sp1 SitesMol. Endocrinol. June 2008; 22(6): p. 1449-1463 [SP1]

Smooth Muscle Expression of Lipoma Preferred Partner Is Mediated by an Alternative Intronic Promoter That Is Regulated by Serum Response Factor/Myocardin, Circ. Res., May 2008; 10.1161/CIRCRESAHA.108.177436 [SRF]


 
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TrueORF : Over-express proteins with fused tags

12,000 Mouse and 25,000 Human ORF clones

The TrueORF product line is the new generation of cDNA clone products based on OriGene's TrueClone Collection. Unlike TrueClones, a TrueORF clone enables expression of the encoded transcript as a tagged protein, which facilitates multiple downstream applications that utilize an anti-tag antibody, such as protein detection, protein purification, subcellular localization, etc.  All TrueORFs are derived from TrueClones using a polymerase with the highest possible fidelity. To further ensure the sequence accuracy, purified plasmids were used as template and a minimal number of PCR cycles was employed to reduce potential PCR mutations. 

All TrueORF inserts are housed in a pCMV6-Entry vector (below) and can be easily shuttled by a simple 'cut-and-paste' mechanism into any of the OriGene PrecisionShuttle Destination Vectors. (see Application Guide)

See full article in our technical journal GeneDex

All TrueORF Human and Mouse ORF clones are provided as 10 ug purified DNA plasmid for immediate application.

Search the entire OriGene TrueORF catalog by Gene Name, Gene Symbol, Accession Number or Keyword in the search box below and select your species of choice

TrueORF vector map:

 pcmventryvector

Features of the TrueORF Entry Vector                                  Download full sequence

  1. The CMV promoter and a Kozak consensus sequence drive protein expression in mammalian cells.
  2. The C-terminus of the ORF is tagged with both Myc and DDK (FlagĀ®)
  3. Ten (10) ug of purified plasmid is provided for immediate transfection and expression experiments.
  4. An antibiotic selection cassette (Neor/Kanr) confers resistance to kanamycin in E. coli and neomycin analogs in mammalian cells.
  5. The T7 promoter upstream of the ORF allows protein expression in cell-free system.
  6. Engineered as the entry vector for the OriGene PrecisionShuttle system.

Entry Vector Feature Table

FlagĀ® is a registered trademark of Sigma-Aldrich



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