Silencing hsp25/hsp27 Gene Expression Augments Proteasome Activity and Increases CD8+ T-Cell–Mediated Tumor Killing and Memory Responses
Cancer Prevention Research, Jan 2012; 5: 122 - 137.
[HSPB1
]
Activation of Androgen Receptor, Lipogenesis, and Oxidative Stress Converged by SREBP-1 Is Responsible for Regulating Growth and Progression of Prostate Cancer Cells
Mol. Cancer Res., Jan 2012; 10: 133 - 142.
[SREBF1
]
Androgen Deprivation Causes Epithelial–Mesenchymal Transition in the Prostate: Implications for Androgen-Deprivation Therapy
Cancer Res., Jan 2012; 72: 527 - 536.
[AR
]
CB2 Cannabinoid Receptors Promote Neural Progenitor Cell Proliferation via mTORC1 Signaling
J. Biol. Chem., Jan 2012; 287: 1198 - 1209.
[Cnr2
]
ORFs cloned in this vector can be expressed in a tunable manner using various dose of IPTG.
Such clones are the best for genes whose over-expression are toxic to the host cells and those whose expression need to be tightly regulated.
The ORF in this vector is expressed as a tagged protein with the C-terminal DDK-His tags. (DDK is the same as FLAG® which is a registered trademark of Sigma Aldrich). The detection and purification of the transgene expression can be achieved by using anti-Myc or anti-DDK antibodies
pTUNE Inducible Vector contains a tunable genetic switch that tightly regulates gene expression in mammalian cells. This switch couples repressor proteins and an RNAi regulation. More details can be found Cell 130, 2007.
