pCMV6-A-GFP

Features:

• This vector is essentially the same as the pCMV6-Entry vector, except for the alternative selection marker (GFP replacing Neomycin). GFP can be used to monitor transfection efficiency or as a cell sorting marker.
• There is no drug selection marker in this vector for mammalian cells.
• The vector itself does not contain additional tag. If the insert is shuttled from pCMV6-Entry via SgfI/AscI and RsrII/MluI, it will express non-tagged protein. If the insert is shuttled from the pCMV6-Entry vector using SgfI/AscI and Pme I, then it will express Myc-DDK tagged protein.

Schematic of the multiple cloning sites:

Download full vector sequence

All OriGene’s TrueORFs inserts can be subcloned into this vector via custom cloning service. To use this service:

1. Find the gene of interest using any of the Gene Name, Gene Symbol, Accession Number or Keyword.
   
   Search:  Human ORF Mouse ORF
2. At the product page displaying the correct gene of interest, choose the appropriate destination vector and select the “Custom Cloning” .