PrecisionShuttle Vectors

The same ORF insert in multiple context for diverse applications

All TrueORF inserts are housed in a pCMV6-Entry vector and can be easily shuttled via PrecisonShuttle, a simple 'cut-and-paste' procedure, into any of the destination vectors. With the wide selection of destination vectors, TrueORF clones are ready-to-use for many downstream protein studies.

The destination vectors offers choices of:

• Epitope tags for protein purification detection
• Fluorescent protein tags for protein visualization
• Various drug marker for stable line establishment
• Inducible expression (pTUNE)
• E.coli expression vectors

The vectors could be ordered as stand-alone products, or as part of a RapidShuttling kit, or as custom shuttling service where OriGene subclones the ORF insert of your interest from the Entry vector into any of these destination vectors.

Destination Vector	SKU	C-Tag	N-Tag	E. coli Selection	Cell Selection	Expression	Price
pCMV6-AC-GFP	PS100010	tGFP		Ampicillin		Mammalian	$320
pCMV6-AN-GFP	PS100019		tGFP	Ampicillin	Neomycin	Mammalian	$320
pCMV6-A-GFP	PS100026			Ampicillin		Mammalian	$320
pCMV6-AC-IRES-GFP	PS100027	Myc-DDK		Ampicillin	Neomycin	Mammalian	$320
pCMV6-AN-RFP	PS100033		tRFP	Ampicillin	Neomycin	Mammalian	$320
pCMV6-AC-RFP	PS100034	tRFP		Ampicillin	Neomycin	Mammalian	$320
pCMV6-AN-YFP	PS100035		tYFP	Ampicillin	Neomycin	Mammalian	$320
pCMV6-AC-YFP	PS100036	tYFP		Ampicillin	Neomycin	Mammalian	$320

Photo gallery of the fluorescent-tagged proteins generated by OriGene with TrueORF clones

Peptide sequence of the DDK-tag (Flag®): N-DYKDDDDK-C
Flag® is a registered trademark of Sigma-Aldrich