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Large Scale Screening via Gain-of-Function
The application of a broad range of cDNAs and derivative products (shRNA, antibodies) enables scientists to better validate and choose targets that are most relevant to disease processes. Some recent publications describing these methods are listed below for your convenience. Large Scale Functional Profiling Using cDNA Clone Collections: A pathway sensor for genome-wide screens of intracellular proteolytic cleavage. Genome Biol. 2008 Apr A functional genomics approach to the mode of action of apratoxin A Nature Chemical Biology.2007 A genomic screen for activators of the antioxidant response element Proc. Natl. Acad. Sci. 2007 Transducer of regulated CREB-binding proteins (TORCs) induce PGC-1 alpha transcription and mitochondrial biogenesis in muscle cells Proc. Natl. Acad. Sci. 2006 Identification of the Wnt signaling activator leucine-rich repeat in Flightless interaction protein 2 by a genome-wide functional analysis High throughput functional genomics: identification of novel genes with tumor suppressor phenotypes. Mol. Cell. Proteomics 2004 (Use of 5,000 genes, including OriGene's TrueClones) Identification of p53 regulators by genome-wide functional analysis Genome-scale functional profiling of the mammalian AP-1 signaling pathway. Identification of a family of cAMP response element-binding protein coactivators by genome-scale functional analysis in mammalian cells. An integrated functional genomics screening program reveals a role for BMP-9 in glucose homeostasis. TIP, a T-cell factor identified using high-throughput screening increases survival in a graft-versus-host disease model. (Use of 8,000 ESTs encoding putative secreted proteins) Large Scale Functional Profiling Using shRNA Expression Constructs: A resource for large-scale RNA-interference-based screens in mammals. A large-scale RNAi screen in human cells identifies new components of the p53 pathway.
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