OriGene - STIM1 (NM_003156) cDNA Clone

OriGene Data

Vector:	pCMV6-XL5	Insert Size: 4000	Restriction Site: NotI-NotI
Sequence Data:	Fully Sequenced ORF 5' Read Nucleotide Sequence 3' Read Nucleotide Sequence
OTI Disclaimer:	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components:	The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Protein Families:	Transmembrane
Protein Pathways:

Reference Data

RefSeq: NM_003156.2, NP_003147	RefSeq Size: 4039	RefSeq ORF: 2058
Synonyms : D11S4896E; GOK; STIM1L
LocusID: 6786	Cytogenetic: 11p15.5
Summary: This gene encodes a type 1 transmembrane protein that mediates Ca2+ influx after depletion of intracellular Ca2+ stores by gating of store-operated Ca2+ influx channels (SOCs). It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region, as well as early hematopoiesis, by mediating attachment to stromal cells. This gene is oriented in a head-to-tail configuration with the ribonucleotide reductase 1 gene (RRM1), with the 3' end of this gene situated 1.6 kb from the 5' end of the RRM1 gene.[provided by RefSeq, Jan 2009].

The use of this clone has been cited in the following citations:

Local Cytosolic Ca2+ Elevations Are Required for Stromal Interaction Molecule 1 (STIM1) De-oligomerization and Termination of Store-operated Ca2+ Entry , Wei-Wei Shen, Maud Frieden, and Nicolas Demaurex, J. Biol. Chem., Oct 2011; 286: 36448 - 36459. [STIM1]

STIM1 couples to ORAI1 via an intramolecular transition into an extended conformation , Martin Muik, Marc Fahrner, Rainer Schindl, Peter Stathopulos, Irene Frischauf, Isabella Derler, Peter Plenk, Barbara Lackner, Klaus Groschner, Mitsuhiko Ikura, et al. , The EMBO Journal (22 March 2011) doi:10.1038/emboj.2011.79 Article [STIM1]

Human Muscle Economy Myoblast Differentiation and Excitation-Contraction Coupling Use the Same Molecular Partners, STIM1 and STIM2, Basile Darbellay, Serge Arnaudeau, Dimitri Ceroni, Charles R. Bader, Stephane Konig, and Laurent Bernheim, J. Biol. Chem., Jul 2010; 285: 22437 - 22447 [STIM1]

Activation of TRPC1 by STIM1 in ER-PM microdomains involves release of the channel from its scaffold caveolin-1, Biswaranjan Pani, Hwei Ling Ong, So-ching W. Brazer, Xibao Liu, Kristina Rauser, Brij B. Singh, and Indu S. Ambudkar, PNAS, Nov 2009; 106: 20087 - 20092 [STIM1]

The molecular architecture of the arachidonate-regulated Ca2+-selective ARC channel is a pentameric assembly of Orai1 and Orai3 subunits, Olivier Mignen, Jill L. Thompson, and Trevor J. Shuttleworth, J. Physiol., Sep 2009; 587: 4181 - 4197 [STIM1]

TRPC channels function independently of STIM1 and Orai1, Wayne I. DeHaven, Bertina F. Jones, John G. Petranka, Jeremy T. Smyth, Takuro Tomita, Gary S. Bird, and James W. Putney, Jr, J. Physiol., May 2009; 587: 2275 - 2298. [STIM1]

Store-operated cyclic AMP signalling mediated by STIM1, Konstantinos Lefkimmiatis, Meera Srikanthan, Isabella Maiellaro, Mary Pat Moyer, Silvana Curci, Aldebaran M. Hofer, Nature Cell Biology 11, 433-442 (15 March 2009) [STIM1]

STIM1- and Orai1-dependent Store-operated Calcium Entry Regulates Human Myoblast Differentiation, Basile Darbellay, Serge Arnaudeau, Stéphane König, Hélène Jousset, Charles Bader, Nicolas Demaurex, and Laurent Bernheim, J. Biol. Chem., Feb 2009; 284: 5370 - 5380. [STIM1]

Stromal Interaction Molecule (STIM) 1 and STIM2 Calcium Sensing Regions Exhibit Distinct Unfolding and Oligomerization Kinetics, Peter B. Stathopulos, Le Zheng, and Mitsuhiko Ikura, J. Biol. Chem., Jan 2009; 284: 728 - 732. [STIM1]

Canonical Transient Receptor Potential 5 Channel in Conjunction with Orai1 and STIM1 Allows Sr2+ Entry, Optimal Influx of Ca2+, and Degranulation in a Rat Mast Cell Line, Hong-Tao Ma, Ze Peng, Takaaki Hiragun, Shoko Iwaki, Alasdair M. Gilfillan, and Michael A. Beaven, J. Immunol., Feb 2008; 180: 2233 - 2239. [STIM1]

Relocalization of STIM1 for activation of store-operated Ca2+ entry is determined by the depletion of subplasma membrane endoplasmic reticulum Ca2+ store, Hwei Ling Ong, Xibao Liu, Krasimira Tsaneva-Atanasova, Brij B Singh, Bidhan C Bandyopadhyay, William D Swaim, James T Russell, Ramanujan S Hegde, Arthur Sherman, and Indu S Ambudkar, J Biol Chem. 2007 Apr 20;282(16):12176-85. [STIM1]

Ca2+ store depletion causes STIM1 to accumulate in ER regions closely associated with the plasma membrane, Minnie M. Wu, JoAnn Buchanan, Riina M. Luik, and Richard S. Lewis, J. Cell Biol. 2006 Sep 11;174(6):803-13. [STIM1]

The elementary unit of store-operated Ca2+ entry: local activation of CRAC channels by STIM1 at ER–plasma membrane junctions, Riina M. Luik, Minnie M. Wu, JoAnn Buchanan, and Richard S. Lewis, J. Cell Biol. 2006 Sep 11;174(6):815-25. [STIM1]

Large Store-operated Calcium Selective Currents Due to Co-expression of Orai1 or Orai2 with the Intracellular Calcium Sensor, Stim1, Jason C. Mercer, Wayne I. DeHaven, Jeremy T. Smyth, Barbara Wedel, Rebecca R. Boyles, Gary S. Bird, and James W. Putney, Jr, J. Biol. Chem., 2006 Aug 25;281(34):24979-90. [STIM1]

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STIM1 Mouse Clones

SKU	Description	Amount	Price	Shipping
MC220402	Mus musculus stromal interaction molecule 1 (Stim1) as transfection-ready DNA	10 ug	$820	6 weeks
MR210034	Myc-DDK-tagged ORF clone of Mus musculus stromal interaction molecule 1 (Stim1) as transfection-ready DNA	10 ug	$820	Next day
MG210034	GFP-tagged ORF clone of Mus musculus stromal interaction molecule 1 (cDNA clone MGC:13964 IMAGE:4191156) in a GFP expression vector	10 ug	$900	3 weeks

STIM1 Rat Clones

SKU	Description	Amount	Price	Shipping
RN212859	NativeORF clone of Rattus norvegicus stromal interaction molecule 1 (Stim1) as transfection-ready DNA	10 ug	$823	6 weeks
RR212859	Myc-DDK-tagged ORF clone of Rattus norvegicus stromal interaction molecule 1 (Stim1) as transfection-ready DNA	10 ug	$823	6 weeks

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STIM1 (NM_003156) Human cDNA Clone

The use of this clone has been cited in the following citations:

STIM1 Mouse Clones

STIM1 Rat Clones

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