HFE (NM_000410) Human Untagged Clone

CAT#: SC119914

HFE (untagged)-Human hemochromatosis (HFE), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HFE
• Synonyms: HFE1; HH; HLA-H; MVCD7; TFQTL2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119914 sequence for NM_000410 edited (data generated by NextGen Sequencing)
  ATGGGCCCGCGAGCCAGGCCGGCGCTTCTCCTCCTGATGCTTTTGCAGACCGCGGTCCTG
  CAGGGGCGCTTGCTGCGTTCACACTCTCTGCACTACCTCTTCATGGGTGCCTCAGAGCAG
  GACCTTGGTCTTTCCTTGTTTGAAGCTTTGGGCTACGTGGATGACCAGCTGTTCGTGTTC
  TATGATCATGAGAGTCGCCGTGTGGAGCCCCGAACTCCATGGGTTTCCAGTAGAATTTCA
  AGCCAGATGTGGCTGCAGCTGAGTCAGAGTCTGAAAGGGTGGGATCACATGTTCACTGTT
  GACTTCTGGACTATTATGGAAAATCACAACCACAGCAAGGAGTCCCACACCCTGCAGGTC
  ATCCTGGGCTGTGAAATGCAAGAAGACAACAGTACTGAGGGCTACTGGAAGTACGGGTAT
  GATGGGCAGGACCACCTTGAATTCTGCCCTGACACACTGGATTGGAGAGCAGCAGAACCC
  AGGGCCTGGCCCACCAAGCTGGAGTGGGAAAGGCACAAGATTCGGGCCAGGCAGAACAGG
  GCCTACCTGGAGAGGGACTGCCCTGCACAGCTGCAGCAGTTGCTGGAGCTGGGGAGAGGT
  GTTTTGGACCAACAAGTGCCTCCTTTGGTGAAGGTGACACATCATGTGACCTCTTCAGTG
  ACCACTCTACGGTGTCGGGCCTTGAACTACTACCCCCAGAACATCACCATGAAGTGGCTG
  AAGGATAAGCAGCCAATGGATGCCAAGGAGTTCGAACCTAAAGACGTATTGCCCAATGGG
  GATGGGACCTACCAGGGCTGGATAACCTTGGCTGTACCCCCTGGGGAAGAGCAGAGATAT
  ACGTGCCAGGTGGAGCACCCAGGCCTGGATCAGCCCCTCATTGTGATCTGGGAGCCCTCA
  CCGTCTGGCACCCTAGTCATTGGAGTCATCAGTGGAATTGCTGTTTTTGTCGTCATCTTG
  TTCATTGGAATTTTGTTCATAATATTAAGGAAGAGGCAGGGTTCAAGAGGAGCCATGGGG
  CACTACGTCTTAGCTGAACGTGAGTGA
  
  Clone variation with respect to NM_000410.3
  396 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000410 unedited
  TGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCGCCCCCCAAAAGAA
  GCGGAGATTTAACGGGGACGTGCGGCCAGAGCTGGGGAAATGGGCCCGCGAGCCAGGCCG
  GCGCTTCTCCTCCTGATGCTTTTGCAGACCGCGGTCCTGCAGGGGCGCTTGCTGCGTTCA
  CACTCTCTGCACTACCTCTTCATGGGTGCCTCAGAGCAGGACCTTGGTCTTTCCTTGTTT
  GAAGCTTTGGGCTACGTGGATGACCAGCTGTTCGTGTTCTATGATCATGAGAGTCGCCGT
  GTGGAGCCCCGAACTCCATGGGTTTCCAGTAGAATTTCAAGCCAGATGTGGCTGCAGCTG
  AGTCAGAGTCTGAAAGGGTGGGATCACATGTTCACTGTTGACTTCTGGACTATTATGGAA
  AATCACAACCACAGCAAGGAGTCCCACACCCTGCAGGTCATCCTGGGCTGTGAAATGCAA
  GAAGACAACAGTACTGAGGGCTACTGGAAGTACGGGTATGATGGGCAGGACCACCTTGAA
  TTCTGCCCTGACACACTGGATTGGAGAGCAGCAGAACCCAGGGCCTGGCCCACCAAGCTG
  GAGTGGGAAAGGCACAAGATTCGGGCCAGGCAGAACAGGGCCTACCTGGAGAGGGACTGC
  CCTGCACAGCTGCANCAGTTGCTGGAGCTGGGGAGAGGTGTTTTGGACCAACAGTGCCTC
  CTTTGGTGAAGTGACACATCATGTGACCTCTTCAGTGACCACTCTACGGTGTCGGNCCTT
  GAACTACTANCCCCAGACATCACCATGAAGTGGCTGAAGGATAGCAGCCATGGATGCCAN
  GNAGNTCGAACCTAAGACGTATTGCCCATGGNGGATGGGACCTACCANGGCTGGATACCN
  TNGNCTGTANCCCCTGNAGNAGAGCANANAATACGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000410 unedited
  AACGCGGCCACTATCTAGAGTCGAGNTCTTTTTTTTTTTTTTTTTTTACTTTTTTTTTTT
  GAAAAGGAGTCTCACTTTGTTACCTTAAGCTGGTATTGCATTGTTTTCAAACTTTTTTTT
  ACTGTTATCTTTGTCTTTTGGGTTCATGCCTTTTTTCTGCCTTATTCTTTCTATTACTTG
  GGACTACAGGCGTCCGTCACCACGCCCATTTAATTTTTTGTATTTTTACTAAAAATGGGG
  TTTCACCATGTTACCCATGATGGTCTCGATCTCCTGACCTCGCGATCCACCCGCTTTGGC
  CTCCCAAAGCGCTGGGATTACAGGCGTGAGCCAACGGGCCCGGCCTACTTATCTTTCTTC
  ATTTAAAAAAGAAATGGGGATAATAGCACCTATCTCATAAAATTATTGTAACAAGTGCAT
  GCAGTAATGCATGCAAACACGCGCCCAAAACACTCCGACACCAACTAACTGCCCTTATCA
  TCCTTATCATAATTCCCATTTTCAAAACAAGGAGAGACCAGGTAGAAAATTATTGGGATT
  CTTCAGGGCCGGAATACCAAAGTAGCATTCCAAATGAAAGCACCATTAAACTTCTGCAAT
  CCGTATGACACCCTTCATGCCAATAGAAAATACCACTTTTACAACCCCTTTAAAAATTTC
  CCTCCTACCCCGCTAAAACCCCATCTTCCTCCCACAGATATCCCGAAGCTTCTTTTGGAC
  AAGTGCCTTTTTATTTCCGCAGCGCCAGGCTTATTCATGGGAACCGACATTAAATCACAA
  GGAATAACCCCCAAGCTTTGTGACAAATGAGCCCCGGGAACACAAGAAAAGGGTTCACAG
  GAAAATTTAACGCGCCCCAAAATCGCTCAACCCCGGCGAAAATCCCGTAAGGTTAAACTC
  ACCCCTTCCCAGGGGAAAAATNTTCTTAAAGTGAAAAGAATCCGGAACCCCCAAATAAGG
  GATCCCTTCCAAAAGGAAAGAAACCCATAGGCACTTTTCAGCGCCGGAAAAGAGN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000410
• Insert Size: 2820 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000410.2, NP_000401.1
• RefSeq Size: 2727 bp
• RefSeq ORF: 1047 bp
• Locus ID: 3077
• UniProt ID: Q30201
• Cytogenetics: 6p22.2
• Domains: MHC_I, ig, IGc1
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: The protein encoded by this gene is a membrane protein that is similar to MHC class I-type proteins and associates with beta2-microglobulin (beta2M). It is thought that this protein functions to regulate iron absorption by regulating the interaction of the transferrin receptor with transferrin. The iron storage disorder, hereditary haemochromatosis, is a recessive genetic disorder that results from defects in this gene. At least nine alternatively spliced variants have been described for this gene. Additional variants have been found but their full-length nature has not been determined. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) encodes the longest isoform. CCDS Note: This CCDS ID represents the longest isoform encoded by the HFE gene. This isoform contains both a Class I Histocompatibility antigen (MHC_I) and an Immunoglobulin constant region (IGc) domain.