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Home RNAi siTran 1.0

siTran 1.0: Optimal transfection reagent for siRNA

siTran 1.0 is a brand-new transfection reagent specially for siRNA duplexes in transient transfection, high-throughput screening, etc. siTran 1.0 is also highly effective in transfecting plasmid DNA. siTran user manual

  • Dual purpose reagent—transfect both siRNA duplex and corresponding cDNA clone
  • High transfection efficiency and low cytotoxicity
  • View recent publications of siTran 1.0. Click here

siTran 1.0 Product list (For larger package sizes, please inquire)

Catalog No. Description Price Availability  
TT300001 siTran 1.0 (0.5 ml) $220 In Stock shopping cart
TT300002 siTran 1.0 (1 ml) $340 In Stock shopping cart
TT300003 siTran 1.0 (5 vials @ 1 ml each) $1390 In Stock shopping cart

High transfection efficiency

High transfection efficiency
Fig. 1. HEK293 cells which were 70% confluent plated in a 96- well microtiter plate were transfected with TYE 563-labeled siRNA. Phase contrast (1A, 1B) and fluorescence (1C, 1D) images for the wells with (1A and 1C) or without (1B and 1D) the addition of siTran 1.0 were taken 24 hrs post transfection

High transfection efficiency

Fig. 2. HEK293 cells which were 70% confluent plated in a 96-well microtiter plate were transfected with tGFP plasmid DNA using siTran 1.0 (2A) or Turbofectin 8.0 (2B). Fluorescence images were taken 48 hrs post transfection.

High transfection efficiency

Fig. 3. HEK293T cells co-transfected using siTran 1.0 with GFP-tagged plasmid DNA and red.

Selected citation of siTran 1.0: 

  1. Taxifolin Enhances Andrographolide-Induced Mitotic Arrest and Apoptosis in Human Prostate Cancer Cells via Spindle Assembly Checkpoint Activation Zhang ZR, Al Zaharna M, Wong MM, Chiu SK, Cheung HY. PLoS One. 2013;8(1):e54577
    [Successful transfection of DU145 cells]
  2. Inhibition of the de-myelinating properties of Aicardi-Goutières Syndrome lymphocytes by cathepsin D silencing Pulliero A, Marengo B, Longobardi M, Fazzi E, Orcesi S, Olivieri I, Cereda C, Domenicotti C, Balottin U, Izzotti A. Biochem Biophys Res Commun. 2013 Jan 18;430(3):957-62
    [Successful transfection of lymphocytes]
  3. A possible gene silencing mechanism: Hypermethylation of the Keap1 promoter abrogates binding of the transcription factor Sp1 in lung cancer cells D Guo, B Wu, J Yan, X Li, H Sun, D Zhou Biochem Biophys Res Commun. 2012 Nov 9;428(1):80-5 [Successful transfection of BEAS 2B cells]
  4. Human ß-defensin-2 enhances IFN- and IL-10 production and suppresses IL-17 production in T cells Naoko Kanda, Masahiro Kamata, Yayoi Tada, Takeko Ishikawa, Shinichi Sato, and Shinichi Watanabe J. Leukoc. Biol., Jun 2011; 89: 935 - 944
    [Successful transfection of T-cells]

 

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