OriGene cDNAs cited in recent published Functional Studies

ERdj4 and ERdj5 Are Required for Endoplasmic Reticulum-associated Protein Degradation of Misfolded Surfactant Protein C, Mol. Biol. Cell. June 2008; 19(6): p. 2620-2630 [DNAJB11]

Protein Kinase C{alpha}-Induced Derepression of the Human Luteinizing Hormone Receptor Gene Transcription through ERK-Mediated Release of HDAC1/Sin3A Repressor Complex from Sp1 Sites, Mol. Endocrinol. June 2008; 22(6): p. 1449-1463 [SP1]

Smooth Muscle Expression of Lipoma Preferred Partner Is Mediated by an Alternative Intronic Promoter That Is Regulated by Serum Response Factor/Myocardin, Circ. Res., May 2008; 10.1161/CIRCRESAHA.108.177436 [SRF]

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Mouse MGC-Plus Clone Collection:
Mouse Full-Length cDNA CLONES

OriGene has expanded its TrueClone collection with the addition of a large collection of mouse full-length cDNA clones in a selectable mammalian expression vector. All Mouse Clones are derived from the NIH MGC Clone collection with significant improvement on the vector. The major differencence are summarized below:

Search the entire OriGene TrueClone Mouse catalog by Gene Name, Gene Symbol, Accession Number or Keyword in the search box below

Application Guide

TrueClone Mouse Vector map: download: Cloning map and Vector sequence

KanNeo no sfiisite vector map

For genes cloned with Sfi I sites, the alternative vector (see below) is used .
download: Vector Map and Cloning Map

Features of the TrueClone Mouse Vector

The CMV promoter and a Kozak consensus sequence drive protein expression in mammalian cells.
Ten (10) ug of purified plasmid is provided for immediate transfection and expression experiments.
An antibiotic selection cassette (Kan^r/ Neo^r) confers resistance to kanamycin in E. coli and neomycin analogs in mammalian cells.
The T7 promoter upstream of the ORF allows protein expression in cell-free system.
Each clone contains a fully verified insert sequence.

Example Verification of Protein Over-expression by Western blot

Mousenorthernblot

Mouse MGC-Plus Clone Collection: Mouse Full-Length cDNA CLONES

Mouse MGC-Plus Clone Collection:
Mouse Full-Length cDNA CLONES