OriGene Technologies, Inc.
Register | Shopping Cart | Blast your Gene | Home
Search:    
About FL cDNA Clones RNAi Gene Expression Lysates and Proteins Antibodies Tissue Other Products Tech Support
orange


OriGene cDNA clones in recent publications
A Bifunctional Regulatory Element in Human Somatic Wee1 Mediates Cyclin A/Cdk2 Binding and Crm1-Dependent Nuclear Export Mol. Cell. Biol., Jan 2010; 30: 116 - 130 [WEE1]
c-myb activates CXCL12 transcription in T47D and MCF7 breast cancer cells Acta Biochim Biophys Sin, Jan 2010; 42: 1 - 7 [MYB(TrueORF)]
Interleukin-11 in Endometrial Adenocarcinoma Is Regulated by Prostaglandin F2{alpha}-F-Prostanoid Receptor Interaction via the Calcium-Calcineurin-Nuclear Factor of Activated T Cells Pathway and Negatively Regulated by the Regulator of Calcineurin-1 Am. J. Pathol., Jan 2010; 176: 435 - 445 [RCAN1]
Peroxisome Proliferator-Activated Receptor {gamma} Coactivator-1{alpha} Interacts with the Androgen Receptor (AR) and Promotes Prostate Cancer Cell Growth by Activating the AR Mol. Endocrinol., Jan 2010; 24: 114 - 127 [PPARGC1A(TrueORF)]
View All Citations >>

MegaTran 1.0: the transfection reagent for large volume applications - 10 ml for $1200

MegaTran 1.0 is a brand new non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection, including large scale protein production via transient transfection, high-throughput screening using cDNA arrays or shRNA libraries, etc.      

  • Superior transfection efficiency compared to leading transfection reagents
  • Effective on 293F suspension cells
  • Improved protein production via transient transfection
  • Reduced cytotoxicity
  • Extremely affordable - $1200 for 10 ml!                                                                    

         MegaTran transfection protocol

MegaTran 1.0 Product list

Catalog No. Description Price
TT200002 MegaTran 1.0 (0.5ml), 165 rxns for 24-well plates $75
TT200003 MegaTran 1.0 (2 ml) $270
TT200004 MegaTran 1.0 (5 X 2ml) $1200
TT200005 MegaTran 1.0 (100ml) $9500

For larger package sizes, please inquire.

High transfection efficiency Megatran Figure 1 FACS analysis of GFP expression in CHO-K1 cells transfected with a GFP expression plasmid.  Cells were transfected with 3ug DNA with 9ul transfection reagent. 72 hrs post transfection, CHO-K1 cells were harvest by trypsine and analyzed on FACS Calibur. Y axis indicates number of cells expressing GFP with increasing intensity.  Left panel represents untransfected control.  Transfection efficiency is 95% with MegaTran 1.0 (right panel) and 63% for Lipofectamine2000 (middle panel). 

Transfection of 293F Suspension Cells

  MegaTran Suspension

293F suspension cells were tranfected with different amounts of plasmid coding human-IgG-GPI with MegaTran 1.0 and 293Fectin.  The number of cells expressing surface IgG was analyzed by FACS using anti-human IgG-FITC 72 hours post transfection.
 
In this experiment, MegaTran 1.0 effectively transfected 80% of 293F suspension cells, equivalent to that of 293Fectin.

High transgene protein expression

Combining the high transfection efficiency and low cytotoxicity, MegaTran 1.0 transfected cells produce much higher level of transgene proteins.

 MegaTran Fig2 Higher production chart

Transient transfection with IgG vectors into CHO-K1 cell line. CHO-K1 cells were transfected with plasmids containing two different monoclonal antibody genes.  Day 3 and 6 post transfection, IgG titer in supernatant was measured by Octet.  Note that expression level continues to increase 35-60% between day 3 and day 6 with MegaTran 1.0.

Simple protocol
MegaTran 1.0 works well in media containing antibiotic and anti-mycobacteria agents.  Transfection can be done in media with or without serum.  There is no requirement for media changes.  (Click here to read the application guide).

Manufacturing conditions
MegaTran 1.0 reagents do not contain any protein or animal components.  Each aliquot is packaged using sterile techniques to eliminate any microbial contamination.

Cell spectrum
MegaTran 1.0 has been tested with the following common cell lines: CHO; HEK293; 293F suspension cells; Hela; COS-7; NIH3T3; MEF; U2OS.

OriGene welcomes experimental results from our customers. Please submit your data with MegaTran 1.0 and receive a Starbucks gift card.

 

Copyright © 2010 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436


Reproduction of any materials from this website is strictly forbidden without permission.