Home cDNA Transfection Reagents MegaTran 1.0|
MegaTran 1.0: the transfection reagent for large volume applications
MegaTran 1.0 is a brand new non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection, including large scale protein production via transient transfection, high-throughput screening using cDNA arrays or shRNA libraries, etc.
- Superior transfection efficiency compared to leading transfection reagents
- Effective on 293F suspension cells
- Improved protein production via transient transfection
- Reduced cytotoxicity
- Extremely affordable
MegaTran transfection protocol
MegaTran 1.0 Product list
||MegaTran 1.0 (0.5ml), 165 rxns for 24-well plates
||MegaTran 1.0 (2 ml)
||MegaTran 1.0 (5 X 2ml)
||MegaTran 1.0 (100ml)
For larger package sizes, please inquire.
High transfection efficiency FACS analysis of GFP expression in CHO-K1 cells transfected with a GFP expression plasmid. Cells were transfected with 3ug DNA with 9ul transfection reagent. 72 hrs post transfection, CHO-K1 cells were harvest by trypsine and analyzed on FACS Calibur. Y axis indicates number of cells expressing GFP with increasing intensity. Left panel represents untransfected control. Transfection efficiency is 95% with MegaTran 1.0 (right panel) and 63% for Lipofectamine2000 (middle panel).
Transfection of 293F Suspension Cells
293F suspension cells were tranfected with different amounts of plasmid coding human-IgG-GPI with MegaTran 1.0 and 293Fectin. The number of cells expressing surface IgG was analyzed by FACS using anti-human IgG-FITC 72 hours post transfection.
In this experiment, MegaTran 1.0 effectively transfected 80% of 293F suspension cells, equivalent to that of 293Fectin.
High transgene protein expression
Combining the high transfection efficiency and low cytotoxicity, MegaTran 1.0 transfected cells produce much higher level of transgene proteins.
Transient transfection with IgG vectors into CHO-K1 cell line. CHO-K1 cells were transfected with plasmids containing two different monoclonal antibody genes. Day 3 and 6 post transfection, IgG titer in supernatant was measured by Octet. Note that expression level continues to increase 35-60% between day 3 and day 6 with MegaTran 1.0.
MegaTran 1.0 works well in media containing antibiotic and anti-mycobacteria agents. Transfection can be done in media with or without serum. There is no requirement for media changes. (Click here to read the application guide).
MegaTran 1.0 reagents do not contain any protein or animal components. Each aliquot is packaged using sterile techniques to eliminate any microbial contamination.
MegaTran 1.0 has been tested with the following common cell lines: CHO; HEK293; 293F suspension cells; Hela; COS-7; NIH3T3; MEF; U2OS; A549.