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OriGene cDNA clones in recent publications
A Bifunctional Regulatory Element in Human Somatic Wee1 Mediates Cyclin A/Cdk2 Binding and Crm1-Dependent Nuclear Export Mol. Cell. Biol., Jan 2010; 30: 116 - 130 [WEE1]

c-myb activates CXCL12 transcription in T47D and MCF7 breast cancer cells Acta Biochim Biophys Sin, Jan 2010; 42: 1 - 7 [MYB(TrueORF)]

Interleukin-11 in Endometrial Adenocarcinoma Is Regulated by Prostaglandin F2{alpha}-F-Prostanoid Receptor Interaction via the Calcium-Calcineurin-Nuclear Factor of Activated T Cells Pathway and Negatively Regulated by the Regulator of Calcineurin-1 Am. J. Pathol., Jan 2010; 176: 435 - 445 [RCAN1]

Peroxisome Proliferator-Activated Receptor {gamma} Coactivator-1{alpha} Interacts with the Androgen Receptor (AR) and Promotes Prostate Cancer Cell Growth by Activating the AR Mol. Endocrinol., Jan 2010; 24: 114 - 127 [PPARGC1A(TrueORF)]

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GFC-Transfection Arrays
(Genome Wide Full-Length cDNA) Arrays

The post genomic area introduced many novel and exciting genome-wide assay platforms, including high-throughput gain-of-function screening, a rapidly emerging technique for identifying genes (see white paper and publications) that are involved in biological processes. OriGene introduced a growing number of GFC-Transfection Arrays as a revolutionary tools for this high-throughput functional screening method.

Our current GFC-Transfection Arrays cover a number of areas including:

GFC Transfection Array Sets Screening Results Number of Genes in Array
Transcription Factors Reporter Assays 704
Apoptosis Genes

Cell Survival

352
Protein Kinases

Luciferase Reporter

352
Secreted Proteins I

Viability Assay

352
Secreted Proteins II

Viability Assay

352
Transmembrane Genes

Viability Assay

352
GPCR Genes

Viability Assay

286
Cell Cycle

Viability Assay

352
Epigenetics I

Viability Assay

352
Epigenetics II

Viability Assay

336
Chaperones 115
Oncogenes 291
Tumor Suppressors 352
Proteases 352
Ubiquitin 264
DNA Repair

Viability Assay

263

Our coverage is always expanding based upon customer's input

GFC-Transfection Arrays are sets of CMV-driven expression ready individual cDNA clones arrayed in both 384 or 96 well formats (click on the corresponding Number of Genes in Array). Each clone is from OriGene’s TrueClone collection of full-length human cDNA clones and each clone is available separately for immediate shipment. GFC-Transfection Arrays are standardized, easy to use and ready for assay. With this product, researchers are no longer restricted to study one gene at a time, but can functionally screen thousands of genes (up to the entire genome) in fast, inexpensive and convenient gain-of-function assays.

The procedure is very simple, just add your reporter, transfection agent and cells, and score the array after 48 hours.

GFCProcess

GFC-Transfection Arrays can be used to study a wide range of biological processes:

  • Gene function
  • Transcriptional regulation
  • Association to specific pathway
  • Cell and tissue-specific gene expression
  • Cell proliferation and differentiation
  • Developmental gene regulation
  • Apoptosis
  • Host-pathogen interactions
  • Sensitivity to drugs and small molecules.


GFC-Transfection Arrays are adaptable for high-throughput readout assays that include:

  • Reporter gene assays
  • Receptor activation assays (e.g., G-protein coupled receptor assays)
  • Nuclear translocations of protein markers
  • Enzymatic assays
  • Immunoassays
  • Protein interaction assays
  • Cell proliferation assays
  • Apoptosis assays
  • Cell toxicity assays
  • Morphological assays

Application Guide

Terms of Use for GFC-Transfection Arrays


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