OriGene cDNAs cited in recent published Functional Studies

ERdj4 and ERdj5 Are Required for Endoplasmic Reticulum-associated Protein Degradation of Misfolded Surfactant Protein C, Mol. Biol. Cell. June 2008; 19(6): p. 2620-2630 [DNAJB11]

Protein Kinase C{alpha}-Induced Derepression of the Human Luteinizing Hormone Receptor Gene Transcription through ERK-Mediated Release of HDAC1/Sin3A Repressor Complex from Sp1 Sites, Mol. Endocrinol. June 2008; 22(6): p. 1449-1463 [SP1]

Smooth Muscle Expression of Lipoma Preferred Partner Is Mediated by an Alternative Intronic Promoter That Is Regulated by Serum Response Factor/Myocardin, Circ. Res., May 2008; 10.1161/CIRCRESAHA.108.177436 [SRF]

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GFC-Transfection Arrays
(Genome Wide Full-Length cDNA) Arrays

The post genomic area introduced many novel and exciting genome-wide assay platforms, including high-throughput gain-of-function screening, a rapidly emerging technique for identifying genes (see white paper and publications) that are involved in biological processes. OriGene introduced a growing number of GFC-Transfection Arrays as a revolutionary tools for this high-throughput functional screening method.

Our current GFC-Transfection Arrays cover a number of areas including:

GFC Transfection Array Sets	Screening Results	Number of Genes in Array
Transcription Factors	Reporter Assays	704
Apoptosis Genes	Cell Survival	352
Protein Kinases	Luciferase Reporter	352
Secreted Proteins I	Viability Assay	352
Secreted Proteins II	Viability Assay	352
Transmembrane Genes	Viability Assay	352
GPCR Genes	Viability Assay	286
Cell Cycle	Viability Assay	352
Epigenetics I	Viability Assay	352
Epigenetics II	Viability Assay	336
Chaperones		115
Oncogenes		291
Tumor Suppressors		352
Proteases		352
Ubiquitin		264
DNA Repair	Viability Assay	263
Screening Set Human I		5,000
Screening Set Human II		3,000
Screening Set Mouse I		5,000

Our coverage is always expanding based upon customer's input

GFC-Transfection Arrays are sets of CMV-driven expression ready individual cDNA clones arrayed in both 384 or 96 well formats (click on the corresponding Number of Genes in Array). Each clone is from OriGene’s TrueClone collection of full-length human cDNA clones and each clone is available separately for immediate shipment. GFC-Transfection Arrays are standardized, easy to use and ready for assay. With this product, researchers are no longer restricted to study one gene at a time, but can functionally screen thousands of genes (up to the entire genome) in fast, inexpensive and convenient gain-of-function assays.

The procedure is very simple, just add your reporter, transfection agent and cells, and score the array after 48 hours.

GFCProcess

GFC-Transfection Arrays can be used to study a wide range of biological processes:

Gene function
Transcriptional regulation
Association to specific pathway
Cell and tissue-specific gene expression
Cell proliferation and differentiation
Developmental gene regulation
Apoptosis
Host-pathogen interactions
Sensitivity to drugs and small molecules.

GFC-Transfection Arrays are adaptable for high-throughput readout assays that include:

Reporter gene assays
Receptor activation assays (e.g., G-protein coupled receptor assays)
Nuclear translocations of protein markers
Enzymatic assays
Immunoassays
Protein interaction assays
Cell proliferation assays
Apoptosis assays
Cell toxicity assays
Morphological assays

Application Guide

GFC-Transfection Arrays (Genome Wide Full-Length cDNA) Arrays

GFC-Transfection Arrays
(Genome Wide Full-Length cDNA) Arrays