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KINASE CLONESET Protein kinases play key roles in most aspects of cellular process, including cell division, signal transduction, apoptosis and cell mobility. Because dysfunction of kinase genes results in a variety of diseases, such as cancer, diabetes and heart disease, this family of genes has captured academic and pharmaceutical research interest. With the completion of the human genome projects, scientists have been able to catalogue the "kinome" - the entire family of kinase genes in human genome. According to a recent survey (Manning, G. et al., Science 298:1912, 2002), the human "kinome" (complete kinase collection) contains 518 gene loci, with multiple alternative splicing variants for some of the loci, and represents 1.7% of human gene repertoire. The OriGene Kinase CloneSet consists of 564 full-length human protein kinase cDNA clones representing 460 gene loci. All genes in Kinase CloneSet are cloned in expression vectors directly downstream of a CMV promoter and are ready for protein expression in mammalian cells. Each clone has been sequenced from the 5' and 3' ends to infer the inclusion of the initiation and stop codons. For those genes sharing multiple splicing variants at the same locus, the variable regions are sequence-verified to qualify a clone to a particular reference. In some cases, OriGene identified variants different than those described in the public database. With no public reference available, such sequences are annotated with a reference number from the same locus, and marked with an asterisk "*". The comprehensive nature of the Kinase CloneSet and the uniformity and expression-readiness of the cloning vector enable comprehensive functional studies on this gene family.
Download Kinase CloneSet Fact Sheet
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