OriGene cDNAs in recent publications

a5ß1-Integrin controls ebolavirus entry by regulating endosomal cathepsins, PNAS, May 2009; 106: 8003 - 8008. [CTSB (CATB)]

BIOPHYSICS AND COMPUTATIONAL BIOLOGY: Crystal structure of human aquaporin 4 at 1.8 Å and its mechanism of conductance, PNAS, May 2009; 106: 7437 - 7442. [AQP4]

Charting the molecular network of the drug target Bcr-Abl, PNAS, May 2009; 106: 7414 - 7419. [SHC1]

Chordin-like 1 and Twisted Gastrulation 1 Regulate BMP Signaling following Kidney Injury, Barry W. Larman, Michele J. Karolak, Derek C. Adams, and Leif Oxburgh, J. Am. Soc. Nephrol., May 2009; 20: 1020 - 1031. [CHRDL1]

Competitive binding of musclin to natriuretic peptide receptor 3 with atrial natriuretic peptide, Shunbun Kita, Hitoshi Nishizawa, Yosuke Okuno, Masaki Tanaka, Atsutaka Yasui, Morihiro Matsuda, Yukio Yamada, and Iichiro Shimomura, J. Endocrinol., May 2009; 201: 287 - 295. [NPR1]

Selective regulation of long-form calcium-permeable AMPA receptors by an atypical TARP Nature Neuroscience 12, 277 - 285 (01 Mar 2009), doi: 10.1038/nn.2266 [CACNG7(TARP7)]

View all citations on TrueClone

FlexClone Collection

The study of gene function is critically dependent on the availability of gene expression vectors suitable for protein production. OriGene has long provided every member of its TrueClone collection of full-length cDNA clones in the pCMV vector suitable for transient expression in mammalian cells. We are now offering our TrueClones in other expression vectors based upon the robust shuttle vector platform from Promega called Flexi® Vector. These FlexClones can be located through the search bar above.

The initial OriGene FlexClones are offered in Flexi® Vector GST (engineered for protein purification) and neoCMV constructs (optimized for stable cell line generation).

The Flexi® Vector is a open-license restriction based cloning system which offer efficient, high-fidelity transfer between multiple expression vector cloning backbones without the need for additional sequence verification. The Flexi ^(a,b,c)Systems use two rare cutting restriction enzymes Sgf I ^(d) and Pme I^(c) in a simple yet powerful directional cloning method for protein coding sequences. These systems provide a rapid efficient and high fidelity way to transfer between a variety of Flexi vectors. Flexi vectors carry the lethal barnase gene which is replaced by the DNA fragment of interest and acts as positive selection for ligations of the insert.

To transfer your protein coding region from one Flexi Vector to another choose the appropriate acceptor vector with the desired expression and tag options the donor and acceptor vectors are digested with the Flexi enzyme blend simultaneously prior to ligation of the insert transformation and selection of cells.

FlexSystem

Figure 1: Transferring protein-coding regions in the Flexi Vector Systems. The Flexi Vector Systems use a flexible directional cloning method for expressing protein coding regions with or without peptide fusion tags The features necessary for expression and any fusion tag are carried on the vector backbone and the protein-coding region can be shuttled between vectors using two rare cutting restriction enzymes Sgf I and Pme I The Flexi Vectors contain a lethal gene, barnase for positive selection of the protein coding sequences and antibiotic resistance marker for selection of colonies containing the Flexi vector

^a)Patent Pending.
^(b)For research use only. Persons wishing to use this product or its derivatives in other fields of use, including without limitation, commercial sale, diagnostics or therapeutics, should contact Promega Corporation for licensing information.
^(c)Licensed under U.S. Pat. No. 5,945,288.
^(d)U.S. Pat. No. 5,391,487.

For information on Flexi® Vectors and related applications for the functional characteriztion of protein-coding regions, please visit the Promega site.