OriGene cDNAs in recent publications

BAT3 and SET1A form a complex with CTCFL/BORIS to modulate H3K4 histone dimethylation and gene expression, Mol. Cell. Biol. Nov.2008; 28(21): p. 6720-6729 [BAT3]

Heme Oxygenase-1 Regulates Cardiac Mitochondrial Biogenesis via Nrf2-Mediated Transcriptional Control of Nuclear Respiratory Factor-1, Circ. Res., Oct 2008; 10.1161/01.RES.0000338597.71702.ad. [AKT1]

Interactions of S100A2 and S100A6 with the Tetratricopeptide Repeat Proteins, Hsp90/Hsp70-organizing Protein and Kinesin Light Chain, J. Biol. Chem., Oct 2008; 283: 28246 - 28258. [TOR1A]

Significance of Organic Cation Transporter 3 (SLC22A3) Expression for the Cytotoxic Effect of Oxaliplatin in Colorectal Cancer, Drug Metab. Dispos., Nov 2008; 36: 2299 - 2306. [POU5F1(OCT3)]

Stroma-dependent apoptosis in clonal hematopoietic precursors correlates with expression of PYCARD Blood, Oct 2008; 10.1182/blood-2008-04-152686. [TNFRSF1A]

MegaTran 1.0: the transfection reagent for large volume applications - 10 ml for $1200

MegaTran 1.0 is a brand new non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection, including large scale protein production via transient transfection, high-throughput screening using cDNA arrays or shRNA libraries, etc.

Superior transfection efficiency compared to leading transfection reagents
Effective on 293F suspension cells
Improved protein production via transient transfection
Reduced cytotoxicity
Extremely affordable - $1200 for 10 ml!

MegaTran transfection protocol

MegaTran 1.0 Product list

Catalog No.	Description	Price
TT200002	MegaTran 1.0 (0.5ml), 165 rxns for 24-well plates	$75
TT200003	MegaTran 1.0 (2 ml)	$270
TT200004	MegaTran 1.0 (5 X 2ml)	$1200
TT200005	MegaTran 1.0 (100ml)	$7500

For larger package sizes, please inquire.

High transfection efficiency FACS analysis of GFP expression in CHO-K1 cells transfected with a GFP expression plasmid. Cells were transfected with 3ug DNA with 9ul transfection reagent. 72 hrs post transfection, CHO-K1 cells were harvest by trypsine and analyzed on FACS Calibur. Y axis indicates number of cells expressing GFP with increasing intensity. Left panel represents untransfected control. Transfection efficiency is 95% with MegaTran 1.0 (right panel) and 63% for Lipofectamine2000 (middle panel).

Transfection of 293F Suspension Cells

293F suspension cells were tranfected with different amounts of plasmid coding human-IgG-GPI with MegaTran 1.0 and 293Fectin. The number of cells expressing surface IgG was analyzed by FACS using anti-human IgG-FITC 72 hours post transfection.

In this experiment, MegaTran 1.0 effectively transfected 80% of 293F suspension cells, equivalent to that of 293Fectin.

High transgene protein expression

Combining the high transfection efficiency and low cytotoxicity, MegaTran 1.0 transfected cells produce much higher level of transgene proteins.

MegaTran Fig2 Higher production chart

Transient transfection with IgG vectors into CHO-K1 cell line. CHO-K1 cells were transfected with plasmids containing two different monoclonal antibody genes. Day 3 and 6 post transfection, IgG titer in supernatant was measured by Octet. Note that expression level continues to increase 35-60% between day 3 and day 6 with MegaTran 1.0.

Simple protocol
MegaTran 1.0 works well in media containing antibiotic and anti-mycobacteria agents. Transfection can be done in media with or without serum. There is no requirement for media changes. (Click here to read the application guide).

Manufacturing conditions
MegaTran 1.0 reagents do not contain any protein or animal components. Each aliquot is packaged using sterile techniques to eliminate any microbial contamination.

Cell spectrum
MegaTran 1.0 has been tested with the following common cell lines: CHO; HEK293; 293F suspension cells; Hela; COS-7; NIH3T3; MEF; U2OS.

OriGene welcomes experimental results from our customers. Please submit your data with MegaTran 1.0 and receive a Starbucks gift card.