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Over 1000 citations of OriGene cDNA clones
A new monoclonal antibody against human alpha-dystroglycan reveals reduced core protein in some, but not all, dystroglycanopathy patients Neuromuscular Disorders Sep 2014 [DAG1]

An extracellular-matrix-specific GEF-GAP interaction regulates Rho GTPase crosstalk for 3D collagen migration Nat. Cell Biol. Sep 2014 []

Cell cycle restriction is more important than apoptosis induction for RASSF1A tumor suppression J. Biol. Chem. Sep 2014 [RASSF5]

Distinct Roles of Ape1 Protein, An Enzyme Involved in DNA Repair, in High or Low Linear Energy Transfer Ionizing Radiation-induced Cell Killing J. Biol. Chem. Sep 2014 [APEX1]

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TurboFectin 8.0 is optimal for shRNA delivery

Double the knockdown efficiency!

hushlink

HEK 293T cells were plated at 10,000 cells/well in a 96-well plate in DMEM with 10% FBS. Both  pCMV-luciferase and HuSH 29-mer shRNA-Luc were transfected using optimal ratios of DNA to each transfection reagent. Luciferase activity was measured at 48hrs. using BriteLite substrate (Perkin Elmer). Results are shown as percent silencing acheived for triplicate experiments.

 

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