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Over 1000 citations of OriGene cDNA clones
MAC-T Cells as a Tool to Evaluate Lentiviral Vector Construction Targeting Recombinant Protein Expression in Milk Anim. Biotechnol. Apr 2015 [IL2]

Angiopoietin-like protein 2 induces androgen-independent and malignant behavior in human prostate cancer cells Oncol. Rep. Jan 2015 [ANGPTL2]

ApoE-isoform-dependent cellular uptake of amyloid-ß is mediated by lipoprotein receptor LR11/SorLA Biochem. Biophys. Res. Commun. Jan 2015 [SORL1]

PCH-2 regulates Caenorhabditis elegans lifespan AGING Jan 2015 [TRIP13]

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TurboFectin 8.0 is optimal for shRNA delivery

Double the knockdown efficiency!

hushlink

HEK 293T cells were plated at 10,000 cells/well in a 96-well plate in DMEM with 10% FBS. Both  pCMV-luciferase and HuSH 29-mer shRNA-Luc were transfected using optimal ratios of DNA to each transfection reagent. Luciferase activity was measured at 48hrs. using BriteLite substrate (Perkin Elmer). Results are shown as percent silencing acheived for triplicate experiments.

 

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