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Over 1000 citations of OriGene cDNA clones
GFP-tagged Apolipoprotein E: a useful marker for the study of hepatic lipoprotein egress Traffic 2017 [CANX]

Aldehyde dehydrogenase activity plays no functional role in stem cell-like properties in anaplastic thyroid cancer cell lines Endocrine 2016 [ALDH1A3]

Asymmetric binding and metabolism of polyunsaturated fatty acids (PUFAs) by CYP2J2 epoxygenase Biochemistry 2016 [CYP2J2]

Copy number variation as a genetic basis for heterotaxy and heterotaxy-spectrum congenital heart defects Philos. Trans. R. Soc. Lond., B, Biol. Sci. 2016 [PFKP]

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TurboFectin 8.0 is optimal for shRNA delivery

Double the knockdown efficiency!

hushlink

HEK 293T cells were plated at 10,000 cells/well in a 96-well plate in DMEM with 10% FBS. Both  pCMV-luciferase and HuSH 29-mer shRNA-Luc were transfected using optimal ratios of DNA to each transfection reagent. Luciferase activity was measured at 48hrs. using BriteLite substrate (Perkin Elmer). Results are shown as percent silencing acheived for triplicate experiments.

 

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