Human Mycoplasma IgG ELISA kit

CAT#: EA100955

Human Mycoplasma IgG ELISA kit

Specifications

Product Data

• Format: 8x12 divisible strips
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3 hours
• Signal: Colorimetric
• Sample Type: Serum
• Sample Volume: 10 µl/well
• Specificity: This kit is used for quantitative detection human Mycoplasma IgG
• Reactivities: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • 1. Microwells coated with M. pneumoniae antigen: 12x8x1
  • 2. Sample Diluent: 1 bottle (ready to use): 22 ml
  • 3. Calibrator: 1 Vial (ready to use): 1ml
  • 4. Positive Control: 1 vial (ready to use): 1ml
  • 5. Negative Control: 1 vial (ready to use): 1ml
  • 6. Enzyme conjugate: 1 bottle (ready to use): 12ml
  • 7. TMB Substrate: 1 bottle (ready to use): 12ml
  • 8. Stop Solution: 1 bottle (ready to use): 12ml
  • 9. Wash concentrate 20X: 1 bottle: 25ml
• Background: Mycoplasma pneumoniae is a pathogen with spectrum of clinical presentations ranging from asymptomatic to pronounced pneumonia. Symptoms start from 6 to 32 days after exposure with headache, malaise, cough, sore throat and fever. The illness can last from a few days to a month or more. Detection by ELISA of M. pneumoniae IgM antibodies or demonstration of a significant increase of specific IgG antibodies is strong evidence for recent infection in the appropriate clinical setting. Specific IgM antibodies typically increase significantly 1 week after clinical onset and specific IgG levels rise in the second week. M. pneumoniae IgM can, however, persist for more than two years after infection, and therefore, detection of specific IgM does not accurately indicate the time of infection. Primary infection and reinfection may be distinguished by the presence of elevated specific IgA and of specific IgM in primary infections and by the presence of elevated specific IgA in the absence of specific IgM in reinfections. In general, the absence of specific IgM in serum collected 10-20 days after onset is strong evidence against primary pneumonia due to M. pneumoniae.