Human Chlamydia pneumonia IgM ELISA kit

CAT#: EA100932

Human Chlamydia pneumonia IgM ELISA kit

Specifications

Product Data

• Format: 8x12 divisible strips
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3 hours
• Signal: Colorimetric
• Sample Type: Serum
• Sample Volume: 10 µl/well
• Specificity: This kit is used for quantitative detection human Chlamydia pneumonia IgM
• Reactivities: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • 1. Microwells coated with C. pneumoniae antigen: 12x8x1
  • 2. Sample Diluent: 1 bottle (ready to use): 22 ml
  • 3. Calibrator: 1 Vial (ready to use): 1ml
  • 4. Positive Control: 1 vial (ready to use): 1ml
  • 5. Negative Control: 1 vial (ready to use): 1ml
  • 6. Enzyme conjugate: 1 bottle (ready to use): 12ml
  • 7. TMB Substrate: 1 bottle (ready to use): 12ml
  • 8. Stop Solution: 1 bottle (ready to use): 12ml
  • 9. Wash concentrate 20X: 1 bottle: 25ml
• Background: Chlamydia pneumoniae, the third recognized of five possible species of Chlamydia (trachomatis, psittaci, pneumoniae, pecorum and an as-yet-unnamed species) was formerly known as Chlamydia spp. strain TWAR. This respiratory pathogen which causes acute respiratory disease, pneumonia and pharyngitis is often isolated from patients with otitis media with effusion, pneumonia with pleural effusion and in asymptomatic respiratory tract infections. C. pneuomoniae causes up to 10% of community-acquired pneumonia cases and it is also a risk factor for coronary heart disease and Guillain-Barré syndrome. Seroprevalence of C.pneumoniae among children is low and increases sharply in teenagers, continues to increase until middle age, and remains high (>50%) into old age, suggesting that most people have more than one C.pneumoniae infection during their lifetime. Primary Chlamydia infection is characterized by a predominant IgM response within 2 to 4 weeks and a delayed IgG and IgA response within 6 to 8 weeks. After acute C.pneumoniae infection, IgM antibodies are usually lost within 2 to 6 months IgG antibody titers rise and usually decrease slowly; whereas IgA antibodies tend to disappear rapidly. When primary Chlamydia infection is suspected, the detection of IgM is highly diagnostic. In reinfection, IgM level may be rarely detected while IgG and IgA levels rise quickly, often in one to two weeks. IgA antibodies have shown to be a reliable immunological marker of primary, chronic and recurrent infections. These antibodies usually decline rapidly to baseline levels following treatment and eradication of the Chlamydia infections.