Mouse Tissue factor/F3 ELISA Kit

CAT#: EA100826

Mouse Tissue factor/F3 ELISA Kit


USD 641.00

2 Weeks*

Size
    • 1 x 96 wells

Product Images

Specifications

Product Data
Assay Type Sandwich ELISA kit of Quantitative Detection for Mouse Tissue factor/F3
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 15.6pg/ml-1000pg/ml
Sample Type Cell culture supernates, serum, plasma(heparin, EDTA, citrate) and Cell lysates.
Sample Volume 100 ul
Specificity This kit is used for quantitative detection of mouse Tissue factor
Sensitivity <10pg/ml
Reactivities Mouse
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
Components
  • 96-well plate precoated with anti-mouse F3 antibody | 1
  • Lyophilized recombinant mouse F3 standard | 10 ng/tube x 2
  • Biotinylated anti-mouse F3 antibody | 100 ul (dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) | 100 ul (dilution 1:100)
  • Sample diluent buffer | 30 ml
  • Antibody diluent buffer | 12 ml
  • ABC diluent buffer | 12 ml
  • TMB color developing agent | 10 ml
  • TMB stop solution | 10 ml
  • Adhesive cover | 4
  • Wash Buffer (25x) | 20 ml
Background Tissue factor, also called platelet tissue factor, factor III, thrombokinase, or CD142 is a protein present in sub endothelial tissue and leukocytes necessary for the initiation of thrombin formation from the zymogene prothrombin. Tissue factor gene was mapped to 1pter-p21 by study of somatic cell hybrids with a species-specific sensitive chromogenic assay. This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII.
Gene Symbol F3
Gene ID 14066

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.