Mouse Neuropilin-1 ELISA Kit

CAT#: EA100807

Mouse Neuropilin-1 ELISA Kit

Specifications

Product Data

• Assay Type: Sandwich ELISA kit of Quantitative Detection for Mouse Neuropilin-1
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 780pg/ml-50,000pg/ml
• Sample Type: Cell culture supernates, serum, and plasma(heparin, EDTA).
• Sample Volume: 100 ul
• Specificity: This kit is used for quantitative detection of mouse Neuropilin-1
• Sensitivity: <20pg/ml
• Reactivities: Mouse
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • 96-well plate precoated with anti- mouse Neuropilin-1 antibody 1
  • Lyophilized recombinant mouse Neuropilin-1 standard 50ng/tubex2
  • Biotinylated anti- mouse Neuropilin-1 antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30 ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
• Background: NRP1(Neuropilin 1) also known as NP1, NRP, BDCA4 or VEGF165R, is a membrane-bound coreceptor to a tyrosine kinase receptor for both vascular endothelial growth factor (VEGF) and semaphorin family members. NRP1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration, and invasion. By somatic cell hybrid analysis, the NRP1 gene was mapped to chromosome 10. NRP1 bounds PGF1 with lower affinity. NRP1-mediated interactions are a necessary element in the initiation of the primary immune response and offer another example, like that of agrin, of a molecule shared by neurologic and immunologic synapses. After T-cell contact with DC, T-cell NRP1 colocalized with CD3 in the immunologic synapse and, sometimes, also at the opposite pole of the T cell. Soluble NRP1 interacts in a homophilic fashion with NRP1 on both DC and T cells, and this binding can be inhibited by blocking antibodies to NRP1. Furthermore, selective NRP1 inhibition in this model suppressed neovascular formation substantially.
• Gene Symbol: Nrp1
• Gene ID: 18186