Human CD21/CR2 ELISA Kit

CAT#: EA100789

Human CD21/CR2 ELISA Kit

Specifications

Product Data

• Assay Type: Sandwich ELISA kit of Quantitative Detection for Human CD21/CR2
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 0.78ng/ml-50ng/ml
• Sample Type: Cell culture supernates, serum and plasma(heparin, EDTA).
• Sample Volume: 100 ul
• Specificity: This kit is used for quantitative detection of human CD21
• Sensitivity: <20pg/ml
• Reactivities: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • 96-well plate precoated with anti- human CD21 antibody 1
  • Lyophilized recombinant human CD21 standard 50ng/tubex2
  • Biotinylated anti- human CD21 antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30 ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
• Background: Complement receptor type 2(CR2), also known as C3DR or CD21, is a protein that in humans is encoded by the CR2 gene. It is mapped to 1q32.2. CR2 is involved in the complement system. It binds to iC3b(inactive derivative of C3b), C3dg, or C3d. CR2 is the membrane protein on B lymphocytes to which the Epstein-Barr virus (EBV) binds during infection of these cells. CR2, together with CD19, CD81, and CD225, forms the B-cell coreceptor complex, which lowers the activation threshold of the B-cell antigen receptor. Genetic variations in this gene are associated with susceptibility to systemic lupus erythematosus type 9 (SLEB9).
• Gene Symbol: CR2
• Gene ID: 1380