Mouse Renin-1 ELISA Kit

CAT#: EA100739

Mouse Renin-1 ELISA Kit

Specifications

Product Data

• Assay Type: Sandwich ELISA kit of Quantitative Detection for Mouse Renin-1
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 62.5pg/ml-4000pg/ml
• Sample Type: Cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
• Sample Volume: 100 ul
• Specificity: This kit is used for quantitative detection of mouse Renin-1
• Sensitivity: <10pg/ml
• Reactivities: Mouse
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • 96-well plate precoated with anti- mouse Renin-1 antibody | 1
  • Lyophilized recombinant mouse Renin-1 standard | 10ng/tubex2
  • Biotinylated anti- mouse Renin-1 antibody | 130ul (dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) | 130ul (dilution 1:100)
  • Sample diluent buffer | 30 ml
  • Antibody diluent buffer | 12ml
  • ABC diluent buffer | 12ml
  • TMB color developing agent |10ml
  • TMB stop solution | 10ml
  • Plate Sealers | 4 piece
  • Wash Buffer powder | 1 Pack
• Background: Renin-1 is also known as Ren, Angiotensinogenase or Kidney rennin. It is a member of peptidase A1 family. Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. It associated to membranes via binding to ATP6AP2.
• Gene Symbol: Ren1
• Gene ID: 19701