Human soluble CD5 ELISA Kit

CAT#: EA100720

Human soluble CD5 ELISA Kit

Specifications

Product Data

• Assay Type: Sandwich ELISA kit of Quantitative Detection for Human soluble CD5
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 15.6 pg/ml- 1000 pg/ml
• Sample Type: Cell culture supernates, Cell lysates, serum and plasma (heparin, EDTA).
• Sample Volume: 100 ul
• Specificity: This kit is used for quantitative detection of human CD5
• Sensitivity: <10pg/ml
• Reactivities: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • 96-well plate precoated with anti-human CD5 antibody | 1
  • Lyophilized recombinant human CD5 standard | 10ng/tube x 2
  • Biotinylated anti-human CD5 antibody | 100 ul (dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) | 100 ul (dilution 1:100)
  • Sample diluent buffer | 30ml
  • Antibody diluent buffer | 12ml
  • ABC diluent buffer | 12ml
  • TMB color developing agent | 10ml
  • TMB stop solution | 10ml
  • Plate Sealers | 4
  • Wash Buffer (25x) | 20 ml
• Background: CD5, also known as T1 or LEU1, is a cluster of differentiation found on a subset of IgM-secreting B cells called B-1 cells, and also on T cells. It is mapped to 11q12.2. Although CD5 can be found on both T cells and B cells, T cells express higher levels of CD5 than B cells. CD5 was used as a T-cell marker until monoclonal antibodies against CD3 were developed. CD5 is upregulated on T cells upon strong activation. In the thymus, there is a correlation with CD5 expression and strength of the interaction of the T cell towards self-peptides. Nevertheless, CD5 serves to mitigate activating signals from the BCR so that the B-1 cells can only be activated by very strong stimuli(such as bacterial proteins) and not by normal tissue proteins.
• Gene Symbol: CD5
• Gene ID: 921