Human Seprase/FAP ELISA Kit

CAT#: EA100645

Human Seprase/FAP ELISA Kit

Product Images

Human Seprase/FAP ELISA Kit standard curve

Specifications

Product Data

• Assay Type: Sandwich ELISA kit of Quantitative Detection for Human Seprase/FAP
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 62.5pg/ml-4000pg/ml
• Sample Type: Cell culture supernates, serum and plasma(heparin, EDTA).
• Sample Volume: 100 ul
• Specificity: This kit is used for quantitative detection of human Seprase
• Sensitivity: <10pg/ml
• Reactivities: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • 96-well plate precoated with anti- human Seprase antibody | 1
  • Lyophilized recombinant human Seprase standard: 10ng/tube | 2
  • Biotinylated anti- human Seprase antibody: 100ul (dilution 1:100) | 1
  • Avidin-Biotin-Peroxidase Complex(ABC) 100ul (dilution 1:100) | 1
  • Sample diluent buffer 30 ml | 1
  • Antibody diluent buffer 12ml | 1
  • ABC diluent buffer 12ml | 1
  • TMB color developing agent 10ml | 1
  • TMB stop solution 10ml | 1
  • Plate Sealers | 4
  • Wash Buffer (25x) 20ml | 1
• Background: FAP(Fibroblast Activation Protein, Alpha) also known as FAPA or SEPRASE, is an inducible cell surface glycoprotein that was originally identified in cultured fibroblasts using monoclonal antibody F19. The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. The FAP gene is mapped on 2q24.2. FAP is most closely related to DPPIV and they share about 50% of their amino acids. FAP is catalytically active as a 170kD dimer and has dipeptidase and gelatinase activity. Its gelatinase activity requires a glycine in P2 position.FAP-alpha shows 48% amino acid identity with dipeptidyl peptidase IV and 30% identity with DPP4-related protein. Northern blot analysis detected a 2.8-kb FAP-alpha mRNA in fibroblasts. Depletion of FAP-expressing cells, which made up only 2% of all tumor cells in established Lewis lung carcinomas, caused rapid hypoxic necrosis of both cancer and stromal cells in immunogenic tumors by a process involving interferon-gamma and tumor necrosis factor-alpha.
• Gene Symbol: FAP
• Gene ID: 2191