Human Angiostatin K1-3 ELISA Kit

CAT#: EA100568

Human Angiostatin K1-3 ELISA Kit

Specifications

Product Data

• Assay Type: Sandwich ELISA kit of Quantitative Detection for Human Angiostatin K1-3
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 156pg/ml-10,000pg/ml
• Sample Type: serum, plasma and Cell culture supernates.
• Sample Volume: 100 ul
• Specificity: This kit is used for quantitative detection of human Angiostatin K1-3
• Sensitivity: <10pg/ml
• Reactivities: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • 96-well plate precoated with anti- human Angiostatin K1-3 antibody 1
  • Lyophilized recombinant human Angiostatin K1-3 standard 10ng/tubex2
  • Biotinylated anti- human Angiostatin K1-3 antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
• Background: Ang K1-3 is a single, non-glycosylated polypeptide chain containing 259 amino acids. It represents a proteolytic fragment of plasminogen containing the first three kringle structures. Ang K1-3 reduces endothelial cell proliferation and acts as a potent inhibitor of angiogenesis and tumor growth. It displays increased inhibitory activity(ED50 =70 nM) relative to kringles 1-4(ED50 = 135 nM).
• Gene Symbol: PLG
• Gene ID: 5340