Human IL-1 beta ELISA Kit

CAT#: EA100263

Human IL-1 beta ELISA Kit

Product Images

Representative standard curve for Human IL-1 beta PicoKine ELISA Kit

Specifications

Product Data

• Assay Type: Sandwich ELISA kit of Quantitative Detection for Human IL-1 beta
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 3.9pg/ml-250pg/ml
• Sample Type: Cell culture supernates, serum and plasma(heparin, EDTA, citrate).
• Sample Volume: 100 ul
• Specificity: This kit is used for quantitative detection of human IL-1 beta
• Sensitivity: <0.15pg/ml
• Reactivities: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • 96-well plate precoated with anti- human IL-1 beta antibody 1
  • Lyophilized recombinant human IL-1 beta standard 1ng/tube x 2
  • Biotinylated anti- human IL-1 beta antibody 100ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 100ul(dilution 1:100)
  • Sample diluent buffer 30ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
  • Plate Sealers 4 x
  • Wash Buffer (25x) 20ml
• Background: Interleukin-1beta(IL-1beta) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1beta, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B(NFKB)-regulated genes. Furthermore, Microenvironmental IL-1beta and, to a lesser extent, IL-1alpha are required for in vivo angiogenesis and invasiveness of different tumor cells. Additional, the cooperation of IL-1beta and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture. Moreover, the association with disease may be explained by the biologic properties of IL-1beta, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.
• Gene Symbol: IL1B
• Gene ID: 3553
• Standard Curve:
  
  Representative standard curve for Human IL-1 beta PicoKine ELISA Kit