12 Lipoxygenase (ALOX12) Mouse Monoclonal Antibody [Clone ID: OTI1C3]

CAT#: TA807718

ALOX12 mouse monoclonal antibody, clone OTI1C3 (formerly 1C3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ALOX12 (Cat# RC210211, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALOX12 (Cat# TA807718)(1:2000). Positive lysates LY424567 (100ug) and LC424567 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-ALOX12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA807718) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-ALOX12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA807718) (1:150)

Specifications

Product Data

• Clone Name: OTI1C3
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ALOX12 (NP_000688) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 75.5 kDa
• Gene Name: arachidonate 12-lipoxygenase, 12S type
• Database Link:
  NP_000688
  Entrez Gene 239 Human
  UniProt ID P18054
• Synonyms: 12-LOX; 12S-LOX; LOG12

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Arachidonic acid metabolism, Metabolic pathways