Cytochrome P450 2C9 (CYP2C9) Mouse Monoclonal Antibody [Clone ID: OTI1D7]

CAT#: TA503796

CYP2C9 (Cytochrome P450 2C9) mouse monoclonal antibody, clone OTI1D7 (formerly 1D7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CYP2C9 (Cat# RC220997, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYP2C9(Cat# TA503796). Positive lysates LY400262 (100ug) and LC400262 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CYP2C9 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CYP2C9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503796)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-CYP2C9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503796)

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  Anti-CYP2C9 mouse monoclonal antibody (TA503796) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CYP2C9 (RC220997).

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  HEK293T cells transfected with either RC220997 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CYP2C9 antibody (TA503796), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI1D7
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CYP2C9(NP_000762) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.53 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 55.4 kDa
• Gene Name: cytochrome P450 family 2 subfamily C member 9
• Database Link:
  NP_000762
  Entrez Gene 1559 Human
  UniProt ID P11712
• Background: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by rifampin. The enzyme is known to metabolize many xenobiotics, including phenytoin, tolbutamide, ibuprofen and S-warfarin. Studies identifying individuals who are poor metabolizers of phenytoin and tolbutamide suggest that this gene is polymorphic. The gene is located within a cluster of cytochrome P450 genes on chromosome 10q24. [provided by RefSeq]
• Synonyms: CPC9; CYP2C; CYP2C10; CYPIIC9; P450IIC9

Reference Data

• Protein Families: Druggable Genome, P450
• Protein Pathways: Arachidonic acid metabolism, Drug metabolism - cytochrome P450, Linoleic acid metabolism, Metabolic pathways, Metabolism of xenobiotics by cytochrome P450, Retinol metabolism