Noxa (PMAIP1) Mouse Monoclonal Antibody [Clone ID: 114C307.1]

CAT#: TA336673

Mouse Monoclonal Noxa Antibody (114C307.1)

Product Images

Immunocytochemistry/Immunofluorescence: Noxa Antibody (114C307.1) - Non-Recombinant Monoclonal TA336673 - ICC/IF analysis using the azide-free version of TA336673 at 1:10. HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). Image objective 40X.

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  Flow Cytometry: Noxa Antibody (114C307.1) - Non-Recombinant Monoclonal TA336673 - Flow cytometry after an intracellular stain was performed on HeLa cells with Noxa [114C307.1] antibody TA336673AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.

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  Immunohistochemistry-Paraffin: Noxa Antibody (114C307.1) - Non-Recombinant Monoclonal TA336673 - Bouin-fixed, paraffin-embedded Chronic Lymphocytic Leukemia (CLL) xenograft stained with Noxa antibody (1:2000), peroxidase-conjugate and DAB chromogen. Staining for 2 hr at RT. Image using the azide-free form of this antibody.

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  Flow Cytometry: Noxa Antibody (114C307.1) - Non-Recombinant Monoclonal TA336673 - Flow cytometry after an intracellular stain was performed on MCF7 cells with Noxa [114C307.1] antibody TA336673AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.

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  Immunohistochemistry-Paraffin: Noxa Antibody (114C307.1) - Non-Recombinant Monoclonal TA336673 - Bouin-fixed, paraffin-embedded Chronic Lymphocytic Leukemia (CLL) xenograft stained with Noxa antibody (1:2000), peroxidase-conjugate and DAB chromogen. Staining for 2 hr at RT. Image using the azide-free form of this antibody.

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  Flow Cytometry: Noxa Antibody (114C307.1) - Non-Recombinant Monoclonal TA336673 - Flow cytometry after an intracellular stain was performed on MCF7 cells with Noxa [114C307.1] antibody TA336673AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.

• Flow Cytometry: Noxa Antibody (114C307.1) - Non-Recombinant Monoclonal TA336673 - Flow cytometry after an intracellular stain was performed on HeLa cells with Noxa [114C307.1] antibody TA336673AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.

• Flow Cytometry: Noxa Antibody (114C307.1) - Non-Recombinant Monoclonal TA336673 - An intracellular stain was performed on Ntera2 cells with Noxa Antibody [114C307.1] TA336673 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).

• Flow Cytometry: Noxa Antibody (114C307.1) - Non-Recombinant Monoclonal TA336673 - An intracellular stain was performed on Ntera2 cells with Noxa Antibody [114C307.1] TA336673 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).

• Western Blot: Noxa Antibody (114C307.1) - Non-Recombinant Monoclonal TA336673 - Western blot analysis in RL-7 cells (a follicular lymphoma).

• Western Blot: Noxa Antibody (114C307.1) - Non-Recombinant Monoclonal TA336673 - Western blot analysis in RL-7 cells (a follicular lymphoma).

• Doc counteracts the ABT-737-mediated increase in Mcl-1 protein.Western blot showing that treatment of LNCaP and PC3 cells with 1 µM ABT-737 (A) increases Mcl-1 but treatment with 1 nM Doc (D) decreases Mcl-1. Combination of Doc + ABT-737 (DA) decreases Mcl-1. In LNCaP, there is less Bcl-2 (D, 24 h) and Bak (DA, 48 h) but few differences in Bcl-xL, Bax, Bid, and Noxa. In PC3, there are few differences in Bcl-2, Bcl-xL, Bax, Bak, Bid, and Noxa.

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Specifications

Product Data

• Clone Name: 114C307.1
• Applications: FC, ICC/IF, IHC, WB
• Recommended Dilution: Flow Cytometry: 1 ug / million cells, Western Blot: 1-2 ug/ml, Immunocytochemistry/ Immunofluorescence: 1:20-1:1000, Immunohistochemistry: 1:10-1:500, Immunohistochemistry-Paraffin: 5 ug/ml
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1, kappa
• Clonality: Monoclonal
• Immunogen: Noxa Antibody was developed by immunizing mice with a fusion protein containing human Noxa.
• Formulation: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: phorbol-12-myristate-13-acetate-induced protein 1
• Database Link:
  NP_066950
  Entrez Gene 58801 MouseEntrez Gene 5366 Human
  UniProt ID Q13794
• Background: Recently, a BH-3 only member of the Bcl-2 family have been identified in human and mouse and designated as Noxa (for damage) (1). The expression of the Noxa gene involves direct activation of its promoter by p53. Increased expression of Noxa protein occurs in normal thymocytes but not in p53-deficient thymocytes. Noxa cDNA codes for a 103-amino acid protein. The coimmunoprecipitation data suggest that Noxa protein may interact with proteins belonging to the Bcl-2 family, such as, Bcl-XL and Mcl-1. Oda et al (1) have also shown that blocking the endogenous Noxa induction results in the suppression of apoptosis. Treatment of cells with Noxa antisense oligonucleotides blocks radiation-induced apoptosis suggesting that Noxa may represent a mediator of p53-dependent apoptosis.
• Synonyms: APR; NOXA
• Note: Immunocytochemistry and Immunofluorescence; See Yakovlev et all (2003) for details.

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: p53 signaling pathway