RANKL (TNFSF11) Mouse Monoclonal Antibody [Clone ID: 12A380]

CAT#: TA336475

Mouse Monoclonal TRANCE/TNFSF11/RANK L Antibody (12A380)

Product Images

Immunohistochemistry-Frozen: TRANCE/TNFSF11/RANK L Antibody (12A380) TA336475 - Analysis in RAW cells using 2 ug of antibody. Shaded histogram represents cells without antibody; green represents isotype control antibody; purple represents antibody. Image using the PE format of this antibody.

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  Flow Cytometry: TRANCE/TNFSF11/RANK L Antibody (12A380) TA336475 - Analysis using the Alexa Fluor (R) 647 conjugate of TA336475. Staining of RANKL in untreated mouse splenocytes (green) and 72 hour ConA-stimulated mouse splenocytes (red) using this antibody. 1 ug of RANKL antibody was used for this assay.

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  Flow Cytometry: TRANCE/TNFSF11/RANK L Antibody (12A380) TA336475 - Analysis using the Alexa Fluor (R) 488 conjugate of TA336475. Staining of RANKL in untreated mouse splenocytes (green) and 72 hour ConA-stimulated mouse splenocytes (red) using this antibody. 1 ug of RANKL antibody was used for this assay.

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  Western Blot: TRANCE/TNFSF11/RANK L Antibody (12A380) TA336475 - Analysis of recombinant RANKL (partial) probed with RANKL antibody at 1 ug/ml.

Specifications

Product Data

• Clone Name: 12A380
• Applications: FC, ICC/IF, IHC, WB
• Recommended Dilution: Immunohistochemistry: 1:10-1:500, Immunohistochemistry-Paraffin: 1:50 - 1:250, Flow Cytometry: 1:10-1:1000, Western Blot: 4-6 ug/ml, Flow (Cell Surface), Immunohistochemistry-Frozen: 1:10-1:500, Immunocytochemistry/ Immunofluorescence
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1, kappa
• Clonality: Monoclonal
• Immunogen: A bacterially expressed fusion protein containing amino acid residues 1-317 of mouse TRANCE was used as immunogen.
• Formulation: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: tumor necrosis factor superfamily member 11
• Database Link:
  NP_003692
  Entrez Gene 21943 MouseEntrez Gene 8600 Human
  UniProt ID O14788
• Background: TRANCE/OPGL/RANKL/ODF is a recently identified member of tumor necrosis factor family. TRANCE (TNF-related activation-induced cytokine receptor) or RANKL {receptor activator of NF-kB (RANK) ligand} has been implicated in interactions between T cells and dendritic cells. RANK ligand (RANKL/TRANCE) binds to RANK on dendritic cells, upregulates the expression of anti-apoptotic protein BcL-XL suggesting a role in dendritic cell survival. TRANCE/RANKL is also important in T- and B-cell maturation. As OPGL/ODF (Osteoprotegerin Ligand/Osteoclast differentiation factor), the same protein can both activate mature osteoclasts and mediate osteoclastogenesis. OPGL/TRANCE deficient mice show severe osteoporesis and complete absence of osteoclasts as a result of lack of osteogenesis.
• Synonyms: CD254; hRANKL2; ODF; OPGL; OPTB2; RANKL; sOdf; TRANCE
• Note: Flow Cytometry (Surface): please see Yoneda et al. 2004 for details. Immunohistochemistry-Frozen: please see Yoneda et al. 2004 for details.

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction