Kcnn1 Rabbit Polyclonal Antibody

CAT#: TA328945

Rabbit polyclonal Anti-KCa2.1 (SK1, SKCa1)

Product Images

Western blot analysis of rat brain cortex: 1. Anti-KCa2.1 (SK1, SKCa1) antibody, (1:200). 2. Anti-KCa2.1 (SK1, SKCa1) antibody, preincubated with the control peptide antigen.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:200-1:2000
• Reactivities: Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide (C)DRPGSGKPPTVSHRLGHRR corresponding to amino acid residues 75-93 of rat KCa2.1 . Intracellular, N-terminal part.
• Formulation: Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
• Reconstitution Method: Add 50 ul double distilled water (DDW) to the lyophilized powder.
• Purification: Affinity purified on immobilized antigen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: potassium calcium-activated channel subfamily N member 1
• Database Link:
  NP_062186
  Entrez Gene 54261 Rat
  UniProt ID P70606
• Background: KCa2.1 is a member of the Ca2+-activated K+ channels family with small conductance that includes KCa2.2 (SK2) and KCa2.3 (SK3). The channel is voltage insensitive and is activated by intracellular Ca2+ in the submicromolar range. It has a similar topology to that of voltage-dependent K+ channels (KV channels), that is six transmembrane domains and intracellular N- and C-termini. KCa2.1 channel expression is roughly confined to neuronal tissue, although there are some indications that it may be present in non-neuronal tissues as well. The sensitivity of KCa2.1 to the bee venom toxin Apamin is a matter of some controversy. Expression of the human KCa2.1 in mammalian cell lines showed that most cells produce channels that can be blocked by Apamin. However, expression of KCa2.1 in Xenopus oocytes produced channels that were mostly insensitive to apamin. Interestingly, the rat KCa2.1 is unable to form functional channels when expressed alone in either cell lines or oocytes.
• Synonyms: hSK1; KCa2.1; SK; SK1; SKCA1