Slc12a3 Rabbit Polyclonal Antibody

CAT#: TA326587

Rabbit Polyclonal Anti-NCC Antibody

Product Images

Western blot analysis of NCC in rat tissues using a 1:1000 dilution of the antibody

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  IF analysis of NCC in rat kidney tissues, showing using a 1:200 dilution of the antibody

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 1:1000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: AA74-95 (rat), 76-97 (hum)
• Formulation: PBS, 50% glycerol and 0.09% sodium azide
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: solute carrier family 12 member 3
• Database Link:
  NP_062218
  Entrez Gene 6559 HumanEntrez Gene 20497 MouseEntrez Gene 54300 Rat
  UniProt ID P55018
• Background: NCC, a thiazide-sensitive NaCl co-transporter, is found on the apical membrane of the distal convoluted tubule, where it is the principal mediator of Na+ and Cl- reabsorption in this segment of the nephron. It is activated by phosphorylation, and has been implicated in renal NaCl and K+ homeostasis . Regulation of NCC expression and phosphorylation by dietary NACl restriction appears to involve SGK1. In experiments with angiotensin II-infused mice, increased sensitivity to Ang II may involved over-activity of NCC . Therefore, NCC is the target of thiazide diuretics used in the treatment of hypertension . Molecular experiments have also shown that NCC has been detected in the lens cortex, core and fiber cells of a rat .
• Synonyms: FLJ96318; NCCT; TSC
• Note: Detects ~160kDa.