NME2 Rabbit Polyclonal Antibody

CAT#: TA325021

Rabbit polyclonal NME2 Antibody (N-term)

Product Images

NME2 Antibody (F40) (Cat. #TA325021) western blot analysis in HL-60, Jurkat, MCF-7 cell line, mouse kidney and rat stomach tissue lysates (35ug/lane).This demonstrates the NME2 antibody detected the NME2 protein (arrow).

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  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

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  IF image of Hela cell stained with NME2 Antibody (N-term) (Cat#TA325021).Hela cells were incubated with NME2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue). NME2 immunoreactivity is localized to Cytoplasm and Nucleus significantly.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:1000, IHC: 1:50~100, IF: 1:10~50
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This NME2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 25-54 amino acids from the N-terminal region of human NME2.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 17298 Da
• Gene Name: NME/NM23 nucleoside diphosphate kinase 2
• Database Link:
  NP_002503
  Entrez Gene 18103 MouseEntrez Gene 83782 RatEntrez Gene 4831 Human
  UniProt ID P22392
• Synonyms: NDKB; NDPK-B; NDPKB; NM23-H2; NM23B; PUF

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Metabolic pathways, Purine metabolism, Pyrimidine metabolism