Myeloperoxidase (MPO) Rabbit Polyclonal Antibody

CAT#: TA319305

Rabbit polyclonal Myeloperoxidase antibody

Product Images

Anti-Myeloperoxidase [Human Leukocytes] detects multiple MPO subunits and chain combinations by WB. Polyclonal rabbit-anti-Myeloperoxidase was used at a 1:5000 dilution to detect 1.0 ug of human myeloperoxidase.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1:5,000 - 1:20,000, WB: 1:500 - 1:5,000, IP: 1:100
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Myeloperoxidase [Human Leukocytes]
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: myeloperoxidase
• Database Link:
  NP_000241
  Entrez Gene 4353 Human
  UniProt ID P05164
• Synonyms: Myeloperoxidase
• Note: Human myeloperoxidase (MPO) is a dimeric protein composed of two heavy subunits (53 kDa) and two light subunits (15 kDa). Each MPO molecule contains two prosthetic porphyrins which play an important role in the catalytic cycle. Molecular weights for MPO isoforms from pools of normal human samples range from 114,000 to 140,000 daltons reflecting a heterogeneous mixture of isoforms when assayed under non-reducing conditions of SDS-PAGE. Often MPO from a single donor will yield a homogenous preparation reflecting a single isoform. The carbohydrate component of MPO, consisting of mannose, glucose and N-acetylglucosamine residues is 2.5%. MPO is inhibited by azide and other compounds. MPO is stored in primary granules of neutrophils and serves as a bactericidal agent in that MPO catalyzes the production of hypochlorous acid (HOCl), a powerful oxidant. HOCl is derived from chloride ion (Cl-) and hydrogen peroxide (H₂O₂). In a number of inflammatory situations, MPO is released into the extracellular matrix where its measurement can be used as an indication of neutrophil activation.

Reference Data

• Protein Families: Druggable Genome