ING5 Goat Polyclonal Antibody

CAT#: TA319227

Goat polyclonal anti-p28 ING5 antibody

Product Images

WB analysis using Anti-p28 ING5 antibody to detect over expressed Human ING5 present in HeLa cell nuclear extracts. This WB shows reactivity with purified recombinant TAP tagged human ING5 protein (lane 3) and does not recognize TAP tagged ING4 on the same membrane (lane 2). A mock purification in lane 1. The blot was incubated with a 1:500 dilution of the antibody at RT.

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  WB analysis using Anti-p28 ING5 antibody to detect over expressed Human ING5 present in cell extracts. Comparison to a molecular weight marker (not shown) indicates a single band of ~36 kDa corresponding to the expected molecular weight for the recombinant protein. Approximately 10 ug of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1, 500.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1:4,000 - 1:16,000, WB: 1:500 - 1:1,000
• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding aa 127-140 of Human p28 ING5protein (Inhibitor of growth family, member 5). This sequence shows homology to isoforms 1 and 2 for ING5.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: inhibitor of growth family member 5
• Database Link:
  NP_115705
  Entrez Gene 84289 Human
  UniProt ID Q8WYH8
• Synonyms: p28ING5
• Note: p28 ING5 is a tumor suppressor protein similar to ING1 that can interact with TP53, inhibit cell growth, and induce apoptosis. This protein contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. This protein can bind TP53 and EP300/p300, a component of the histone acetyl transferase complex, suggesting its involvement in TP53-dependent regulatory pathway. Multiple alternatively spliced transcript variants have been observed. The accession number listed below is for variant (1) encodes the longest isoform.

Reference Data

• Protein Families: Druggable Genome