UBE2B Rabbit Polyclonal Antibody

CAT#: TA308525

Rabbit Polyclonal antibody to UBE2B (ubiquitin-conjugating enzyme E2B (RAD6 homolog))

Product Images

UBE2B antibody detects UBE2B protein by western blot analysis. A. 30 ug 293T whole cell lysate/extract. B. 30 ug whole cell lysate/extract of 3xFlag-human UBE2B-transfected 293T cells. 12 % SDS-PAGE. UBE2B antibody (TA308525) dilution: 1:5000

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  Sample (30 ug of whole cell lysate). A: NIH-3T3 12% SDS PAGE. TA308525 diluted at 1:1000.

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  Immunofluorescence analysis of paraformaldehyde-fixed A431, using UBE2B (TA308525) antibody at 1:500 dilution.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; WB:1:500-1:10000
• Reactivities: Human, Mouse (Predicted: Rat, Pig, Rabbit, Bovine, Rhesus Monkey)
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment contain a sequence corresponding to a region within amino acids 1 and 152 of UBE2B
• Formulation: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 17 kDa
• Gene Name: ubiquitin conjugating enzyme E2 B
• Database Link:
  NP_003328
  Entrez Gene 22210 MouseEntrez Gene 81816 RatEntrez Gene 7320 Human
  UniProt ID P63146
• Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution. [provided by RefSeq]
• Synonyms: E2-17kDa; HHR6B; HR6B; RAD6B; UBC2
• Note: Seq homology of immunogen across species: Rat (100%), Pig (100%), Rabbit (100%), Rhesus Monkey (100%), Bovine (100%)

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Ubiquitin mediated proteolysis