PNPT1 Rabbit Polyclonal Antibody

CAT#: TA308058

Rabbit Polyclonal antibody to PNPase (polyribonucleotide nucleotidyltransferase 1)

Product Images

Sample (30 ug of whole cell lysate). A: A431. 7.5% SDS PAGE. TA308058 diluted at 1:1000.

• 

  PNPase antibody detects PNPase protein at mitochondria on human normal colon by immunohistochemical analysis. Sample: Paraffin-embedded human normal colon. PNPase antibody (TA308058) dilution: 1:250.

• 

  Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PNPase (TA308058) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with anti-alpha tubulin antibody (Red) at 1:2500.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human (Predicted: X. tropicalis)
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 783 of PNPase (Uniprot ID#Q8TCS8)
• Formulation: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 86 kDa
• Gene Name: polyribonucleotide nucleotidyltransferase 1
• Database Link:
  NP_149100
  Entrez Gene 87178 Human
  UniProt ID Q8TCS8
• Background: PNPT1 is a subunit of the exosome complex, which is involved in 3-prime-to-5-prime exoribonuclease activity for RNA processing and degradation (Raijmakers et al., 2002 [PubMed 12419256]). [supplied by OMIM]
• Synonyms: COXPD13; DFNB70; old-35; OLD35; PNPASE
• Note: Seq homology of immunogen across species: Xenopus tropicalis (80%)

Reference Data

• Protein Pathways: Purine metabolism, Pyrimidine metabolism, RNA degradation