RNF22 (TRIM3) Goat Polyclonal Antibody

CAT#: TA302432

Goat Polyclonal Antibody against BERP / RNF22

Product Images

TA302432 staining (0.05ug/ml) of Mouse Brain lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

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  HEK293 overexpressing TRIM3 (RC211928) and probed with TA302432 (mock transfection in first lane)

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1:64,000. WB: 1-3µg/ml.
• Reactivities: Mouse (Expected from sequence similarity: Human, Rat, Dog, Cow)
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide with sequence AKREDSPGPEVQP-C, from the N Terminus of the protein sequence according to NP_006449.2; NP_150594.2.
• Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: lot specific
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 84461 Da
• Gene Name: tripartite motif containing 3
• Database Link:
  NP_150594
  Entrez Gene 55992 MouseEntrez Gene 83616 RatEntrez Gene 476834 DogEntrez Gene 10612 Human
  UniProt ID O75382
• Background: The protein encoded by this gene is a member of the tripartite motif (TRIM) family, also called the 'RING-B-box-coiled-coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to cytoplasmic filaments. It is similar to a rat protein which is a specific partner for the tail domain of myosin V, a class of myosins which are involved in the targeted transport of organelles. The rat protein can also interact with alpha-actinin-4. Thus it is suggested that this human protein may play a role in myosin V-mediated cargo transport. Alternatively spliced transcript variants encoding the same isoform have been identified. [provided by RefSeq]
• Synonyms: BERP; HAC1; RNF22; RNF97