CD4 Rat Monoclonal Antibody [Clone ID: YKIX302.9]

CAT#: SM409PS

CD4 rat monoclonal antibody, clone YKIX302.9, Purified

Specifications

Product Data

• Clone Name: YKIX302.9
• Applications: FC, IHC
• Recommended Dilution: Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 10⁶ cells or 100 µl whole blood.
  Immunohistochemistry on Frozen Sections.
• Reactivities: Canine
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Canine Concanavilin A activated T-cell blasts.
• Specificity: This antibody Clone YKIX302.9 recognises the Canine CD4 cell surface antigen.
  The antibody has been shown to block MHCII dependent T cell responses in vivo and in vitro. In the Dog CD4 is expressed by neutrophils as well as by lymphocytes subsets.
• Formulation: PBS
  State: Purified
  State: Liquid purfied IgG fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Database Link:
  UniProt ID P33705
• Background: CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.
• Synonyms: T-cell surface antigen T4/Leu-3