TPSAB1 Mouse Monoclonal Antibody [Clone ID: AA1]

CAT#: SM1220PT

TPSAB1 mouse monoclonal antibody, clone AA1, Purified

Product Images

Staining of Human Tonsil with Mouse anti Human Mast Cell Tryptase Antibody

Specifications

Product Data

• Clone Name: AA1
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Western Blot.
  Immunofluorescence.
  Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Paraffin Sections: 1/10,000.
  This product requires either protein digestion pre-treatment of paraffin sections e.g. pronase, or HIER. Sodium citrate buffer pH 6.0 is recommended for this purpose.
  Recommended Positive Control: Tonsil.
• Reactivities: Canine, Feline, Human, Monkey, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human mast cell tryptase purified from Human lung tissue.
  Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
• Specificity: This antiobdy recognises Mast Cell Tryptase, both alpha and beta isoforms.
  This antibody is an excellent marker for mast cells, and does not bind to any other cell type in immunohistology.
• Formulation: PBS, pH 7.4
  State: Purified
  State: Liquid purified IgG fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: tryptase alpha/beta 1
• Database Link:
  Entrez Gene 54271 RatEntrez Gene 7177 Human
  UniProt ID Q15661
• Background: Mast cells contain a number of preformed chemical mediators such as histamine, chymase, carboxypeptidase and proteolytic tryptase.
  Human Mast Cell Tryptase is considered to be an important marker of mast cell activation as well as an important mediator of inflammation.
• Synonyms: TPSB1, Tryptase I, Mast Cell Tryptase

Reference Data

• Protein Families: Druggable Genome, Protease, Secreted Protein