CD4 Mouse Monoclonal Antibody [Clone ID: RPA-T4]

CAT#: SM1058R

CD4 mouse monoclonal antibody, clone RPA-T4, PE

Product Images

Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD4:RPE.

Specifications

Product Data

• Clone Name: RPA-T4
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 10 µl of neat-1/10 diluted antibody to label 10e6 cells or 100 µl of whole blood.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human PHA blasts.
  Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
• Specificity: This antibody recognises CD4. Epitope mapping studies have shown that antibodies, produced by clone RPA-T4, recognise an epitope within domain 1, of the extracellular region, of the CD4 molecule. Clone RPA-T4 has been reported to block gp120-CD4 interaction and inhibit syncytium formation. We recommend the use of SM1058LE for this purpose.
• Formulation: PBS, pH 7.4 containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer.
  Label: PE
  State: Lyophilized purified IgG fraction.
  Label: R. Phycoerythrin (RPE)
• Reconstitution Method: Restore with distilled water
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: PE
• Storage: Prior to and following reconstitution store the antibody at 2-8°C.
  DO NOT FREEZE!
  This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Gene Name: CD4 molecule
• Database Link:
  Entrez Gene 920 Human
  UniProt ID P01730
• Background: CD4 is a 55kD cell surface glycoprotein that is primarily expressed on a subpopulation of T lymphocytes, on peripheral blood monocytes and on tissue macrophages.
• Synonyms: T-cell surface antigen T4/Leu-3