AVD Rabbit Polyclonal Antibody

CAT#: R1201F

AVD rabbit polyclonal antibody, FITC

Specifications

Product Data

• Applications: FC, IHC
• Recommended Dilution: Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays.
• Reactivities: Chicken
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Avidin [Hen Egg White].
• Specificity: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and Avidin.
• Formulation: 0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4 with 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative.
  Label: FITC
  State: Lyophilized purified Ig fraction
  Label: Diaminotriazynlaminofluorescein (DTAF) (Molecular Weight 530 daltons)
  Absorption emission: 495 nm / 528 nm
  Molar radio: 3.0 moles DTAF per mole of Rabbit IgG
• Reconstitution Method: Restore with 2.0 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Immunoaffinity chromatography
• Conjugation: FITC
• Storage: Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
  This antibody is stable for one month at 2-8°C as an undiluted liquid.
  Dilute only prior to immediate use.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: avidin
• Database Link:
  Entrez Gene 396260 Chicken
  UniProt ID P02701
• Background: The biological function of avidin is not known. It is a homotetrameric glycoprotein found in chicken egg white, and forms a strong non covalent specific complex with biotin (one molecule of biotin per subunit of avidin).
• Synonyms: AVD