Trypsinogen Rabbit Polyclonal Antibody

CAT#: R1116HRPS

Trypsinogen rabbit polyclonal antibody, HRP, Purified

Product Images

Western blot of peroxidase conjugated rabbit anti-Trypsinogen primary antibody Cat.-No. [R1116HRP]. Lane 1: Trypsinogen. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Peroxidase rabbit secondary antibody at 1/1,000 for 60 min at RT. Block

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution:
  Western blot: 1/1,000-1/5,000.
  ELISA: 1/10,000-1/40,000.
  This product has been assayed against 1.0 µg of Trypsinogen [bovine pancreas] in a standard capture ELISA using ABTS as a substrate at room temperature. A working dilution of 1/1,000 to 1/3,000 of the reconstitution concentration is suggested for this product.
• Reactivities: Bovine
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Trypsinogen from bovine pancreas
• Specificity: This product detects bovine Trypsinogen. Cross reactivity against Trypsinogen from other tissues and species may occur but have not been specifically determined.
  Immunoelectrophoresis give a single precipitin arc against anti-peroxidase, anti-rabbit serum as well as purified and partially purified Trypsinogen [bovine pancreas].
• Formulation: 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
  Label: HRP
  State: Purified
  State: Lyophilized purified Ig fraction
  Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free)
  Preservative: 0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide)
  Label: Horseradish peroxidase
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: HRP
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.