Alpha 1 Acid Glycoprotein (ORM1) Rabbit Polyclonal Antibody

CAT#: R1044PS

Alpha 1 Acid Glycoprotein (ORM1) rabbit polyclonal antibody, Purified

Product Images

Western blot of ORM1/Alpha-1-acid glycoprotein antibody. [R1044P]. Lane 1: Human AGP1. Load: 50 ng. Primary antibody: Anti human ORM1/AGP1 antibody at 1/1,000 overnight at 4°C. Secondary antibody: HRP conjugated rabbit secondary antibody at 1/40,000 for 30 min at RT. Blocking buffer for 30 min at RT. Predicted at 24 kDa / Observed at 40 kDa; protein migrates at 40 kDa.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/135,000.
  Western blot: 1/2,000-1/10,000.
  Immunohistochemistry: Paraffin (FFPE) or Frozen sections; 1/100 suggested to begin.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Alpha-1-acid glycoprotein (AGP1) from human plasma
• Specificity: This antibody detects human Alpha-1-acid glycoprotein (ORM1 / AGP1).
  Immunoelectrophoresis give a single precipitin arc against anti-rabbit serum as well as purified and partially purified AGP1 from human plasma.
• Formulation: 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
  State: Purified
  State: Lyophilized purified Ig faction
  Preservative: 0.01% Sodium azide
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Multistep process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: orosomucoid 1
• Database Link:
  Entrez Gene 5004 Human
  UniProt ID P02763
• Background: Alpha 1 Acid Glycoprotein (AGP) is an acute phase plasma protein synthesized by the liver. The protein is believed to regulate the interaction between blood cells and endothelial cells, and together with haptoglobin and C reactive protein, also regulates the extravasation of the cells during infection and inflammation. AGP is a major acute phase reactant, both in human and in rat. In human plasma, AGP (44 kDa) is found at levels of 0.5-1.4 mg/ml. Its concentration in blood plasma is elevated during acute inflammation, such as trauma, malignancies, myocardial infarction, rheumatoid arthritis, bacterial infections, after major surgery, in cases of chronic pain etc, and can be used for the diagnosis of inflammatory conditions.
• Synonyms: Orosomucoid-1, OMD1, Alpha-1-acid glycoprotein 1
• Note: Conjugates available. Please ask for details.