PDGF AA (PDGFA) (PDGF-AA) Rabbit Polyclonal Antibody

CAT#: PP1061P1

PDGF AA (PDGFA) (PDGF-AA) rabbit polyclonal antibody, Aff - Purified

Conjugation: Unconjugated Biotin


USD 297.00

2 Weeks*

Size
    • 50 ug

Product Images

Other products for "PDGF AA"

Specifications

Product Data
Applications ELISA, FN, IHC, WB
Recommended Dilution Neutralisation:
To yield one-half maximal inhibition [ND50] of the biological activity of hPDGF-AA (5.0 ng/ml),
a concentration of 0.05 - 0.07 µg/ml of this antibody is required.
Immunohistochemistry:
The antibody stained formalin-fixed, paraffin embedded sections of normal human skin at
1.0 µg/ml overnight at 4°C. (A HRP-labeled polymer detection system was used with a
non-alcohol soluble AEC chromogen and a proteinase K antigen retrieval).
ELISA:
Indirect - To detect hPDGF-AA by indirect ELISA (using 100 μl/well antibody solution),
a concentration of 0.5 - 2.0 μg/ml of this antibody is required; allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPDGF-AA.
Sandwich - To detect hPDGF-AA by sandwich ELISA (using 100 μl/well antibody solution),
a concentration of 0.5 - 2.0 μg/ml of this antibody is required; in conjunction with a biotinylated anti-human PDGF-AA (PP1061B) as the detection antibody at least 0.2 - 0.4 ng/well of recombinant hPDGF-AA can be detected.
Western blot:
To detect hPDGF-AA by Western blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPDGF-AA is 1.5 -3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivities Human
Host Rabbit
Clonality Polyclonal
Immunogen Highly pure (>98%) recombinant hPDGF-AA
Specificity The antibody recognizes the human Platelet Derived Growth Factor AA (hPDGF-AA).
Not tested in other species.
Formulation PBS, pH 7.2, without preservatives
State: Aff - Purified
State: Lyophilized purified Ig fraction
Reconstitution Method Centrifuge vial prior to opening.
Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification Immunoaffinity chromatography using immobilized human PDGF matrix
Conjugation Unconjugated
Storage The lyophilized antibody is stable at -20°C for one year from despatch.
The reconstituted antibody can be stored for two weeks at 2-8°C. Frozen aliquots are stable for six months when stored at -20°C. Avoid repeated freezing and thawing.
Gene Name Homo sapiens platelet derived growth factor subunit A (PDGFA), transcript variant 1
Synonyms Platelet-derived growth factor subunit A, PDGF alpha, PDGF A, PDGF-A, Platelet-derived growth factor alpha, PDGF subunit A, Platelet-derived growth factor A chain, PDGF1, PDGF-1
Reference Data
Protein Families Druggable Genome
Protein Pathways Cytokine-cytokine receptor interaction, Focal adhesion, Gap junction, Glioma, MAPK signaling pathway, Melanoma, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton

{0} Product Review(s)

0 Product Review(s) Submit review

Be the first one to submit a review

Product Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.