GRO gamma (CXCL3) Rabbit Polyclonal Antibody

CAT#: PP1014B1

GRO gamma (CXCL3) rabbit polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: ELISA:
  Direct: To detect hGRO-gamma by direct ELISA (using 100 µl/well antibody solution) this antibody can be used at a concentration of 1.0 µg/ml. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hGRO-gamma.
  Sandwich: To detect hGRO-gamma by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25–1.0 µg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human GRO-gamma (PP1014P1 or PP1014P2) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of recombinant hGRO-gamma.
  Western blot
  To detect hGRO-gamma by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGRO-gamma is 1.5-3.0 ng/lane, under either recombinant hGRO-gamma.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure (>98%) recombinant hGRO-gamma (human GRO-gamma).
• Specificity: Human Growth Related Oncoprotein gamma (GRO gamma).
• Formulation: PBS, pH 7.2 without preservatives.
  Label: Biotin
  State: Lyophilized purified Ig fraction.
  Label: conjugated
• Reconstitution Method: Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity Chromatography.
• Conjugation: Biotin
• Storage: Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Database Link:
  Entrez Gene 2921 Human
  UniProt ID P19876
• Background: Growth Regulated Protein gamma (GRO gamma) is a cytokine that may play a role in inflammation and affects endothelial cells in an autocrine fashion. It belongs to the intercrine alpha family (small cytokine c-x-c) (chemokine cxc).
• Synonyms: C-X-C motif chemokine 3, GRO-gamma, GRO3, GROG, SCYB3, MIP2-beta
• Note: Centrifuge vial prior to opening!