CX3CL1 Rabbit Polyclonal Antibody

CAT#: PP1009P1

CX3CL1 rabbit polyclonal antibody, Aff - Purified

Product Images

Formalin-fixed, paraffin-embedded sections of human pancreas malignant ductal adenocarcinoma using Fractalkine / CX3CL1 Cat.-No [PP1009P]

Specifications

Product Data

• Applications: ELISA, FN, IHC, WB
• Recommended Dilution: Neutralization.
  Sandwich ELISA: To detect Human Fractalkine by Sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5-2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Fractalkine (Cat.-No PP1009B1 or PP1009B2) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human Fractalkine.
  Western Blot: To detect Human Fractalkine by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFractalkine is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
  Immunohistochemistry on Paraffin Sections: 0.25 µg/ml with an overnight incubation at 4°C. This antibody stained formalin-fixed, paraffin-embedded sections of human pancreas malignant ductal adenocarcinoma. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.
  
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure (> 98%) E.coli derived recombinant Human Fractalkine (Cat.-No PA057).
• Specificity: This antibody is specific to Human Fractalkine.
• Formulation: PBS, pH 7.2 without preservatives
  State: Aff - Purified
  State: Lyophilized (sterile filtered) purified Ig fraction
• Reconstitution Method: Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity Chromatography using an immobilized Human Fractalkine matrix
• Conjugation: Unconjugated
• Storage:

  Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: C-X3-C motif chemokine ligand 1
• Database Link:
  Entrez Gene 6376 Human
  UniProt ID P78423
• Background: CX3CL1 is a member of the delta chemokine subfamily that contains a unique CX3C cysteine motif near the N-terminal. Unlike other known chemokines, it is a type 1 membrane protein containing a chemokine domain tethered on a long mucin-like stalk. CX3CL1, a leukocyte chemoattractant, is expressed in various tissues including the brain, heart, lung, kidney, skeletal muscle, and testis. The expression is reported to be up-regulated in endothelial cells and microglia by inflammatory signals. CX3CR1, a specific receptor for fractalkine, mediates both leukocyte migration and adhesion.
• Synonyms: Neurotactin, FKN, NTT, SCYD1
• Note: Centrifuge vial prior to opening!