VEGF Receptor 2 (KDR) Mouse Monoclonal Antibody [Clone ID: KDREWC]

CAT#: DM3503B

VEGF Receptor 2 (KDR) mouse monoclonal antibody, clone KDREWC, Biotin, Purified

Specifications

Product Data

• Clone Name: KDREWC
• Applications: ELISA, FC, WB
• Recommended Dilution: ELISA (1-10 µg/ml)
  FACS analysis and Cell Sorting (2-20 µg/ml).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant Human soluble extracellular KDR protein (D7) (110 kDa)
• Specificity: The monoclonal antibody will detect native Human VEGFR-2/KDR in ELISA experiments and on the surface of different Human cell types.
• Formulation: PBS
  Label: Biotin
  State: Purified
  State: Lyophilized purified IgG fraction from Cell Culture Supernatant
  Stabilizer: 50xBSA
  Preservative: 0.02% Sodium Azide
• Reconstitution Method: Restore in distilled sterile water to 0.1-1.0 mg/ml
• Purification: Protein G Chromatography
• Conjugation: Biotin
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: kinase insert domain receptor
• Database Link:
  Entrez Gene 3791 Human
  UniProt ID P35968
• Background: Endothelial cells express three different vascular endothelial growth factor (VEGF) receptors, belonging to the family of receptor tyrosine kinases (RTKs). They are named VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1), VEGFR-3 (Flt-4). Their expression is almost exclusively restricted to endothelial cells, but VEGFR-1 can also be found on monocytes. All VEGF-receptors have seven immunoglobulin-like extracellular domains, a single transmembrane region and an intracellular split tyrosine kinase domain. VEGFR-2 has a lower affinity for VEGF than the Flt-1 receptor, but a higher signaling activity. Mitogenic activity in endothelial cells is mainly mediated by VEGFR-2 leading to their proliferation. The binding of VEGF165 to VEGFR-2 is dependent on heparin.
• Synonyms: VEGFR2, FLK1, KDR, VEGF Receptor 2