Heparan Sulfate Proteoglycan 2 (HSPG2) Rat Monoclonal Antibody [Clone ID: A7L6]

CAT#: DM3099P

Heparan Sulfate Proteoglycan 2 (HSPG2) rat monoclonal antibody, clone A7L6, Purified

Product Images

Immunohistochemistry on Frozen Section of Human kidney showing strong reactivity in the extracellular matrix and basement membrane.

Specifications

Product Data

• Clone Name: A7L6
• Applications: IF, IHC, IP, WB
• Recommended Dilution: Immunoprecipitation.
  Immunoblotting: 1/100-1/1000.
  Immunocytochemistry.
  Immunohistochemistry on Frozen and Paraffin-Embedded Tissues: 1/25-1/200 with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.
• Reactivities: Bovine, Human, Mouse, Rat, Cod, Wolffish
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: High molecular mass material derived from the Engelbreth-Holm-Swarm (EHS) tumor matrix containing laminin, entactin and HSPG.
• Specificity: A7L6 recognizes domain IV of the core protein of the large Heparan Sulphate Proteoglycan or Perlecan.
  The reactivity is independent of the galactosaminoglycan moieties. Therefore, the epitope is not sensitive to Heparitinase treatment.
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: heparan sulfate proteoglycan 2
• Database Link:
  Entrez Gene 3339 Human
  UniProt ID P98160
• Background: Proteoglycans are macromolecules consisting of a variety of core proteins with covalently attached one or several polysaccharide chains of the glycosaminoglycan type (heparan sulphate, heparin, chondroitin sulphate, dermatan sulphate or keratan sulphate). At least two forms of basement membrane heparan sulphate proteoglycan (HSPG) have been identified. One with a large core protein (> 400 kD) and one with a small core protein (30 kD). The large HSPG is probably the most abundant basement membrane proteoglycan. It is located predominantly in the lamina lucida, where it forms clustered aggregates and interacts with other basement membrane components to form the matrix. In addition, it also plays a critical role in attachment of cells to the basal membrane via integrin receptors.
• Synonyms: PLC, Basement membrane-specific heparan sulfate proteoglycan core protein